PP1γ2对树鼩精子成熟和运动性的调控作用研究

doi:10.16431/j.cnki.1671-7236.2019.05.020

Abstract

Abstract:

In order to elucidate whether PP1γ2 is present or not in tree shrews'spermatozoa and its form in epididymal sperm,and further explore the regulation of PP1γ2 on sperm maturation and motility in tree shrews,the present form and phosphorylation levels of PP1γ2 in caput and cauda epididymal spermatozoa under various conditions have been analyzed by Western blotting.The effects of db-cAMP,IBMX or Ca²⁺ on the phosphorylation levels of PP1γ2 in tree shrew sperm were investigated.The effects of okadaic acid (OA) and calyculin A (CA) on the phosphorylation level of PP1γ2 and motility in caput and cauda epididymal spermatozoa were further studied with tree shrews as experimental animals in the present study.The results showed that PP1γ2 was present in caput and cauda epididymal spermatozoa of tree shrew,and the phosphorylation level of PP1γ2 was far higher in cauda epididymal spermatozoa than that of caput spermatozoa.db-cAMP,IBMX or Ca²⁺ did not change the phosphorylation level of PP1γ2,OA or CA could significantly increase not only the phosphorylation level of PP1γ2 in caput and cauda epididymal spermatozoa but also sperm motility (P<0.05),especially for caput epididymal spermatozoa.The optimal concentrations of OA and CA were 1 μmol/L and 10 nmol/L,respectively,and the optimal incubation time were 15 and 20 min,respectively.This study indicated that PP1γ2 played an important role in regulating sperm maturation and motility in tree shrews through phosphorylation and dephosphorylation.

Key words: tree shrew; PP1γ2; sperm maturation; motility; epididymis

CLC Number:

S814

ZHU Fangfang, LI Xue, YANG Minghua, WU Wenjing, KUANG Haiou, LI Yahui. Study on the Regulation of PP1γ2 on Sperm Maturation and Motility in Tree Shrews[J]. , 2019, 46(5): 1421-1428.

References

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Study on the Regulation of PP1γ2 on Sperm Maturation and Motility in Tree Shrews

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