›› 2019, Vol. 46 ›› Issue (5): 1413-1420.doi: 10.16431/j.cnki.1671-7236.2019.05.019

• Genetics and Breeding • Previous Articles     Next Articles

Developmental Changes of CAPN1 and H-FABP Genes Expression and Their Intramuscular Fat Content in Yellow Feather Broilers Under Free-range

ZHU Mengting, XIE Yifan, WANG Xiaolu, ZHAO Zongsheng   

  1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
  • Received:2018-09-25 Online:2019-05-20 Published:2019-05-20

Abstract:

In order to investigate the relationship between CAPN1,H-FABP genes and the growth and intramuscular fat contentcontent (IMF) of Yellow-feathered broilers under the free-range feeding system,130 3-week-old health Yellow-feathered broiler hens with similar weight were selected as test material and randomly divided into two groups with cage rearing (control group) and free-range group (test group).A total of 360 samples of chest muscles,leg muscles and abdominal fat of Yellow-feathered broiler hens were collected,the CAPN1 and H-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content was determined by the method of Soxhlet extraction.The results showed that the CAPN1 and H-FABP mRNAs of Yellow-feathered broilers were expressed in different degrees in the chest muscles,leg muscles and abdominal fat.With the increase of age,the overall expression trend of CAPN1 in free-range group was increase-decrease-increase-decrease,and the expression level of CAPN1 mRNA in chest and abdominal fat were significantly higher in cage rearing than that in free-range during 3 to 6 w (P<0.05).The overall expression trend of H-FABP decreased with the increasing age,and the expression trends in the chest and leg muscles were the same and they were significantly higher in free-range than the cage rearing (P<0.05).The expression of H-FABP in abdominal fat was higher in cage rearing group (P>0.05).Compared to free-range chickens,cage rearing chickens grew faster,and the rate of abdominal fat and IMF content were higher also.The test results could provide some reference for the CAPN1 and H-FABP genes molecular breeding of Yellow-feathered chickens.

Key words: Yellow-feathered broiler; CAPN1 gene; H-FABP gene; intramuscular fat

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