增强子预测及靶向测序技术研究进展

doi:10.16431/j.cnki.1671-7236.2019.05.007

Abstract

Abstract:

Enhancers have traditionally been defined as genomic cis-regulatory sequences that regulate the transcription of one or more genes independently of the transcription direction and relative position to the target promoter.The activity of enhancers plays a key regulatory role in growth and development,gene expression diversity,evolution and human disease occurrence.Therefore,understanding the functional characteristics of enhancers will not only help us understand the space-time specificity of gene expression,but also help us finally reveal the key biological issues such as cells differentiate,species evolve,and non-coding region variation leads to disease.With the rapid development of high-throughput sequencing technology,the methods and tools for the prediction,identification and functional annotation of enhancers also show diversity,including sequence motif analysis,in vivo binding sites of transcription factors and cofactors,feature chromatin and histone modification,large-scale parallel enhancer analysis,and CRSPR related technologies.This article reviews six methods of enhancer identification,recognition and prediction based on high-throughput sequencing technology at the whole genome level using regulatory factor binding sites,chromatin accessibility,histone modification,enhancer-promoter interaction,eRNAs and in vitro luciferase,and expounds in detail how to target enhancer research using gene editing technology.Finally,the role of enhancer research and individual research enhancer at the whole genome level is prospected,and the methods of targeted prediction of enhancer are described in detail,and the advantages and limitations of each method are compared,providing a reference for further research in this field.

Key words: enhancer; high-throughput sequencing; targeted sequencing; CRISPR

CLC Number:

WANG Jin, ZHAO Zhengwei, MA Lina, MA Qing. Research Progress on Enhancer Forecast and Targeted Sequencing Technology[J]. , 2019, 46(5): 1308-1315.

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Research Progress on Enhancer Forecast and Targeted Sequencing Technology

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