Previous Articles     Next Articles

Establishment and Application of Nested RT-PCR for Detecting Porcine Epidemic Diarrhea Virus

DENGZU Li-ying1, CHEN Lu2   

  1. 1.Zhengzhou Kindergarten Teachers’ College, Zhengzhou 450000, China;2.Henan Agricultural University, Zhengzhou 450002, China)
  • Online:2014-01-20 Published:2014-02-27

Abstract: To detect porcine epidemic diarrhea virus (PEDV) accurately and sensitively, two pairs of specific primers amplifying M gene were designed and the nested RT-PCR method was established. The lowest detective value of the nested RT-PCR as proved by the sensitivity test was 50 fg of PEDV RNA template, however,that of the conventional RT-PCR was 50 pg. The nested RT-PCR primers designed in the study could not be used to amplify the PRRSV, CSFV, TGEV and GARV RNA templates in the specificity test. The results showed that the nested RT-PCR was a rapid, sensitive and cost-effective method for detecting trace amounts of PEDV nucleic acid.

Key words: porcine epidemic diarrhea virus; nested RT-PCR; M gene