Abstract: To identicate Listeria monocytogenes isolates from North of Xingjiang sheep, 8 Listeria monocytogenes isolates of 30 Listeria spps isolates, isolated from diseasing sheep, healthy sheep, environment and bird droppings at sheep farms in epidemic areas of Listeriosis were identified and serotyped by multipelx PCR method. The results showed that among 4 strains of Listeria isolated from diseasing sheep, 3 strains were serovar 1/2a or 4b of Listeria monocytogenes, and 1 strain was not Listeria monocytogenes; 5 strains from healthy sheep serovar were 1/2a;3 strains isolated from water, 2 strains from crow droppings and 16 strains of Listeria monocytogenes isolated from healthy sheep were not isolates of Listeria monocytogenes. The study suggested that there was correlativity among serovars of strains of Listeria monocygenes from diseasing sheep, healthy sheep and reference strain Listeria monocytogenes.

Key words: sheep; multiplex PCR; Listeria spps; Listeria monocytogenes; serovars