副猪嗜血杆菌aroA基因的克隆与序列分析及2种三重PCR检测方法的建立

Abstract

Abstract: In this study, 5 strains of Haemophilus parasuis(Hps)were identified by ‘satellite’ growing test, biochemical and PCR assay. The results showed that aroA gene of the isolated Hps had a high degree of similarity with classical culture strain in the sequence of nucleotide and amino acid sequence, which was between 96.3% to 99.8% and 98.2% to 99.5% respectively. We implemented two program of triplex PCR assay, one group was used to detect simultaneously infections of Hps, PCV2 and PPV, the other group was developed to detect the PCV2, PPV and PRV. The sensitivity tests indicated that the triplex PCR assay may be used for detecting Hps, PPV, PRV and PCV2 of 10⁵ dilute strength; the DNA minimun detectable quantity of Hps, PPV, PRV, PCV2 was 12, 16, 7.6 and 6.3 pg respectively.

Key words: Haemophilus parasuis; triplex PCR assay; aroA gene

CLC Number:

MU He-ping, WANG Xing-mei, YE Ya-qiong, LI Jia-wei, LI Yu-gu. Isolation and Identification of Haemophilus parasuis, Cloning and Sequence Analysis of aroA Gene and Institution of Two Program of Triplex PCR Assay[J]. .

References

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Isolation and Identification of Haemophilus parasuis, Cloning and Sequence Analysis of aroA Gene and Institution of Two Program of Triplex PCR Assay

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