抗猪传染性胃肠炎病毒与猪流行性腹泻病毒卵黄抗体制备及其临床应用研究

Abstract

Abstract: The hyperimmunized yolk antibodies against porcine transmissible gastroenteritis virus (TGEV) and poricine epidemic diarrhoea virus (PEDV) was obtained, and its cure effect was tested. The hens were immunized with the two combined inactivated vaccine of TGEV and PEDV. After the antibody titer reached to 1∶64 tested by double diffusion and eggs of hyperimmunity were collected. The hyperimmunized yolk antibodies were purified by chloroform extraction and salt precipitation with ammonium sulfate. Microorganism detection, safe experiment, artificially infected piglets with the antigen and clinical treatment test were conducted to evaluate the application effect. The cure rate of artificial infection was 100% and the cure rate of clinic treatment test was 88.0%. The antibody had obvious curative effect against TGEV and PEDV.

Key words: TGEV; PEDV; hyperimmunized yolk antibodies; piglet diarrhea

CLC Number:

S858.28

CUI Huan-zhong, JIANG Hai-long, ZHANG Jia-li, ZHANG Hui, QIN Gui-xin. Study and Application the Hyperimmunized Yolk Antibodies of TGEV and PEDV in Piglets[J]. , 2012, 39(6): 173-175.

[1]	SUN Shouhu, CHEN Zhifei, GUO Zhixuan, YU Ting, WANG Xiuwu, LI Songbei, WEI Wenkang, HE Dongsheng. Study on Codon Usage Bias of N gene of Three Porcine Coronaviruses and Their Host Adaptive Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2403-2413.
[2]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[3]	HUO Mingkai, GUAN Feihu, QIU Runhui, WEI Chunyan, YU Haitao, ZHU Jiale, ZHANG Hui. Study on the Mechanism of DDX1 Inhibiting Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1556-1566.
[4]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.
[5]	YANG Zhi, YU Jiaxin, YANG Linfu, WANG Kang, LI Guanzong, DUAN Gang, YANG Fumei, LIU Zhiqi, ZHOU Ke, HE Jun, DAI Feiyan. Study on the Therapeutic Effect of Modified Sini Decoction on Piglet Diarrhea [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1707-1717.
[6]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[7]	WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.
[8]	SONG Shiying, GUO Weilu, XIA Xuefeng, ZHANG Xue, BI Zhenwei, ZHANG Xuehan, FAN Baochao, DONG Hailong, LI Bin. Expression and Purification of Porcine IFN-δ5 and Its Effect Analysis Against PEDV Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3171-3179.
[9]	LIU Yutong, YAO Yanting, LI Ruiling, HAN Wenqi, SHI Ting, WANG Yidan, REN Yupeng. Analysis of Genetic Variation of Porcine Epidemic Diarrhea Virus in Parts of Southwest China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2279-2290.
[10]	WANG Zihang, DENG Xingmei, QIU Runhui, ZHU Dexin, LI Jia, TAO Tingting, ZHU Jiale, SUN Zhihua, ZHANG Hui. Regulation of Long Noncoding RNAs in Autophagy Induced by PEDV Infected Vero-E6 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1942-1950.
[11]	ZHANG Mingliang, GENG Yachun, LIAN Kaiqi, MA Lei, CHANG Ying, WANG Shuangshan, ZHANG Fuliang. Cloning,Bioinformatics Analysis and Truncated Fragment Expression for Truncated M Gene of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1479-1487.
[12]	YAN Zhong, YANG Hanchun. Surveillance and Genetic Variation Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus in Part Provinces and Municipalities of China from 2018 to 2020 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 424-434.
[13]	CHEN Wenguang, ZHANG Yang, XIAO Xiaoyue, LIU Yanyan, CHEN Xueying, WANG Xiumin, QIN Junjie, LI Yanhua. Investigation of the Effective Components and Active Mechanism of Qiweng Huangbo Powder in the Treatment of Piglet Diarrhea Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4898-4908.
[14]	ZHANG Tianai, LI Tingting, TAO Xiaoli, LI Tengfei, LIN Jiafeng, HU Siman, LIU Wenxiu, TONG Wei, LI Yonggang. Prokaryotic Expression of Porcine Epidemic Diarrhea Virus S Protein and Preparation and Identification of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4383-4391.
[15]	BAI Weiqin, KA Chula, WU Zhiyong, MIAO Miao, TA Na, GE Riletu. Expression and Identification of B-cell Epitope Embedded S-layer Protein of Porcine Epidemic Diarrhea Virus S2 Gene in Lactobacillus paracasei [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4004-4011.

Study and Application the Hyperimmunized Yolk Antibodies of TGEV and PEDV in Piglets

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments