China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (5): 1514-1522.doi: 10.16431/j.cnki.1671-7236.2020.05.026

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Investigation on the Infection of Piroplasma in Yak in Ganzi Prefecture of Sichuan Province

LAN Lan1,2, LI Youying2, YANG Danjiao2, PAN Yao2, CHEN Dongming2, HE Anxiang2, TANG Cheng1,3   

  1. 1. College of Life Science and Technology Southwest Minzu University, Chengdu 610041, China;
    2. Animal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding 626000, China;
    3. Sichuan Beef Cattle Innovation Team, Chengdu 610041, China
  • Online:2020-05-20 Published:2020-05-18

Abstract: The purpose of this study was to investigate the infection of Piroplasma in yak in all 18 counties (cities) of Ganzi Tibetan autonomous prefecture (Ganzi prefecture).From June to November 2018,a total of 1 381 whole blood samples of clinical healthy yak were collected in all 18 counties of Ganzi prefecture.A nested PCR targeting 18S rRNA was used to detect Piroplasma.Furthermore,a total of 124 positive PCR products were randomly selected to identify parasite species.The results showed that 438 of 1 381 yak blood samples were detected as Piroplasma positive with an average positive rate of 31.72%.Specifically,the 385 of 1 093 yak blood samples were detected as Piroplasma positive in semi-agricultural and semi-pastoral counties,the average positive rate was 35.22%.53 of 128 yak blood samples were detected as Piroplasma positive in pure pastoral counties,the average positive rate was 18.40%.In 124 positive PCR products,the species infecting yak were Theileria sinensis (76),Theileria luwenshuni (25),Theileria orientalis (3),Theileria ovis (2),Babesia bigemina (1) and Theileria buffeli (1),respectively.The infection rate of Theileria sinensis was 61.29%,and the infection rate of Theileria luwenshuni was 20.16%.The results in this study showed that the infection rate of Piroplasma in yak was high in Ganzi prefecture,and was different among regions,the predominant species were Theileria sinensis and Theileria luwenshuni,contributing to the prevention and control of piroplasmosis in yak in Ganzi prefecture.

Key words: Piroplasma; infection condition; yak

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