China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (5): 1498-1505.doi: 10.16431/j.cnki.1671-7236.2020.05.024

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Isolation and Identification of Psychrotrophic Bacteria on Large-scale Cattle Farms and Analysis of Pollution Sources in Xinjiang Wuchang and Kashgar Areas

LI Jianzhou1, ZHAO Yankun2, WANG Fulan2, CHEN He2, MA Xin1, FAN Xue1, DU Xiaohui1, LI Ming1, SHAO Wei1   

  1. 1. College of Animal Science, Xinjiang Agricultural University, Laboratory of Herbivore Nutrition for Meat and Milk in Xinjiang, Urumqi 830052, China;
    2. Institute of Quality Standard&Testing Technology for Agro-Products, Xinjiang Academy of Agriculture Science, Laboratory of Quality and Safety Risk Assessment for Agro-Products, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, China
  • Online:2020-05-20 Published:2020-05-18

Abstract: The purpose of the experiment was to understand the contamination of the psychrotrophiles in the large-scale cattle farms in the north and south Xinjiang,and to provide scientific basis for the establishment of effective prevention and control measures.Total of 396 samples were collected from 8 sampling points of 6 cattle farms in Wuchang and Kashgar regions under similar conditions of temperature (TM) and relative humidity (HR).GB 4789.2-2016 National Food Safety Standard for Food Microbiological Testing Determination of Total Colonies was used to isolate and culture psychrotrophiles,and BLAST sequence identification was performed.According to the results,276 strains of halophilic bacteria were isolated from Wuchang region and Kashgar region.Through %ID identification,15 dominant psychrophilic bacteria were identified,including Bacillus thuringiensis,Pseudomonas,Bacillus cereus,Bacillus,Acinetobacter,Bacillus circulans,Bacillus licheniformis,Psychrobacter,Streptococcus sanguis,Bacillus megaterium,Arthrobacter,Pseudomonas fluorescens,Streptococcus,Paenibacillus,Exiguobacterium.Compare the differences between the two places,the results showed that the total number of strains isolated in bed soil,feed and air were the most in Wuchang and Kashgar area.The number of chilling bacteria in 7 environmental sites except the environment of the pipeline group in Kashgar area were lower than that in Wuchang area.Among them,the amount of bacteria in the bed soil,milk tank mouth and feed in Kashgar area were extremely significant or significantly lower than that in Wuchang area (P<0.01;P<0.05).The amount of bacteria in the mouth of the pipeline was significantly higher than that in Kashgar (P<0.01).To sum up,under similar conditions of temperature,humidity and illumination,the management level had a great influence on the contamination of psychrophiles in cattle farms in north and south Xinjiang and the corresponding control measures should be established in different regions.This study provided a theoretical basis for exploring the source of raw milk pollution in north and south Xinjiang.

Key words: raw milk; psychrophiles; harm; isolation and identification; traceability

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