抗CP型、NCP型牛病毒性腹泻病毒高免卵黄抗体的制备

›› 2009, Vol. 36 ›› Issue (6): 124-127.

• 疾病防治 • Previous Articles Next Articles

Preparation of IgY against Cytopathic (CP) and Noncytopathic (NCP) Bovine Viral Diarrhea Virus (BVDV)

ZHAO Yu-long^1，2, WU Mo-er³, BO Xin-wen², LI Yan⁴, ZHONG Fa-gang², LI Na ^1，2, KU Chao-feng^1，2

(1.College of Animal Science and Technology, Shihezi University, Shihezi 832003, China; 2.The Breed and Biotechnology Key Laboratory of Sheep in Xinjiang Bingtuan, Shihezi 832000, China; 3.143 Agglomerate of Ziniquan Animal Husbandry 3th Company, Shihezi 832000, China; 4.Animal and Veterinary Station of Xinjiang Production and Construction Group, Urumqi 830063, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
Contact: ZHONG Fa-gang

Abstract

Abstract: Layer hens were immunized with cytopathic (CP) and noncytopathic (NCP) bovine viral diarrhea virus(BVDV), the egg yolk was successfully treated with modified PEG6000 method. The purity of IgY was detected with SDS-PAGE and the IgY titer was assayed by indirected ELISA after 7 days, and the neutralizing antibody titer to NCP BVDV with immunoperoxidase monolayer assay (IPMA). The results suggested that higher purity of lgY was observed with the use of modified PEG6000. We can detect anti-BVDV IgY in the first 14 days. After being immunized with 4 doses of the conjugate, the titer of IgY against CP BVDV was 1∶32000, against NCP BVDV was 1∶40000 and kept the conditions after 3 months. Neutralizing antibody titer to NCP BVDV was 1×10^-3.

Key words: BVDV; IgY; indirect ELISA; non-cell pathogenicity

CLC Number:

S852.4⁺3

ZHAO Yu-long;WU Mo-er;BO Xin-wen;LI Yan;ZHONG Fa-gang;LI Na;KU Chao-feng;. Preparation of IgY against Cytopathic (CP) and Noncytopathic (NCP) Bovine Viral Diarrhea Virus (BVDV)[J]. , 2009, 36(6): 124-127.

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Preparation of IgY against Cytopathic (CP) and Noncytopathic (NCP) Bovine Viral Diarrhea Virus (BVDV)

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