›› 2011, Vol. 38 ›› Issue (5): 97-100.

• 生物技术 • Previous Articles     Next Articles

Tandem Expression of FnbpA,ClfA,Ebps Antigen Epitopes of Staphylococcus aureus and the Preparation of the Polyclonal Antibody

GAO Xiang, XU Hui-hui, LEI Lian-cheng   

  1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China
  • Received:2010-10-03 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract: In order to achieve the special antigen protein and prepare polyclonal antibody,so as to lay the foundation of the rapid detection of Staphylococcus aureus,this experiment screened the antigen epitopes of FnbpA,ClfA,Ebps of Staphylococcus aureus using the DNAStar software,then connected the epitopes with a linker and synthesized the whole genes. The objective gene was cloned into expression vector pGEX-4T-1,followed by transformation into E.coli BL21. The expression production was purified through Sepharose 4B affinity chromatography method,and the purified protein was injected into rabbits to prepare polyclonal antibody,at the 42 days peripheral blood was collected and the serum was obtained. The results revealed that this experiment has successfully established the expression vector,and the antibody titer was more than 1∶10000 analysised by ELISA. These results were benefit to develop the rapid detection of Staphylococcus aureus using immunological methods.

Key words: Staphylococcus aureus; antigen epitopes; tandem expression; polyclonal antibody

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