›› 2011, Vol. 38 ›› Issue (5): 93-96.

• 生物技术 • Previous Articles     Next Articles

Development PCR Assay for Detecting Salmonella pullorum Based on Allele-specific PCR

TU Yu-rong1,2, CHEN Jian-hong2, REN Tao1, ZHANG Ji-pei2, SI Xing-kui2, NIU Sen1,2   

  1. 1. College of Life Science,Foshan University,Foshan 528231,China;2. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
  • Received:2010-11-09 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract: Based on the nuclei polymorphism of rfbS gene sequence of Salmonella pullorum at site 237 and 598 compared with S.gallinarum,a pair of allele-specific primers were designed and synthesized,and an allele-specific PCR method for detecting S.pullorum was developed consistent with result of the serological and biochemical method and the sensitivity of the PCR assay was 18 pg DNA and 4.7×104 CFU/mL cultural liquid.The results showed that the developed allele-specific PCR was a very rapid,sensitive and specific molecular tool for the detection and identification of S.pullorum isolates.

Key words: allele-specific PCR; Salmonella pullorum; rfbS gene

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