›› 2011, Vol. 38 ›› Issue (5): 89-92.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence of MC1R Gene 5'-Flanking Sequence in Rabbit

LUO Hao, HOU Jia-ni, LI Yu-mei, YAN Shou-qing   

  1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China
  • Received:2010-11-15 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract: The rabbit coat color as one of available genetic markers plays an important role in determining breed characteristics, purity, and crossing combination. The melanocortin-1 receptor gene (MC1R) is an important candidate gene for coat color heredity because some coat color changes in many species resulted from the nucletide sequence variants of MC1R gene. In this study,two primers (RMCR1 and RMCR2) were designed to amplify the 5'-flanking region sequences of MC1R from rabbit genome according to the known rabbit MC1R sequence(AM180881). A DNA fragment of 930 bp in length was cloned from 5'-flanking sequence of MC1R gene by an end tailed-PCR strategy, the homology of nucleotide was 99% with the known rabbit MC1R sequence(AM180881).

Key words: rabbit; coat color; MC1R gene

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