内蒙古绒山羊褪黑激素受体1a基因外显子1 克隆及序列分析

›› 2011, Vol. 38 ›› Issue (5): 82-84.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of Exon1 MTNR1a Gene of Inner Mongolian Cashmere Goat

SUN Yong-ming^1,3, ZHANG Yan-jun², LI Guo-hua⁴, LIU Zhi-hong², WANG Zhi-ying², YU Xin-lei², ZHAO Yan-hong²

1. College of Life Science, Inner Mongolia University,Hohhot 010020,China;2. College of Animal Science, Inner Mongolia Agricultural University,Hohhot 010018,China;3. Maodeng Pasture in Xilinhaote, Xilinhaote 026000,China;4. Wengniute County Animal Husbandry Modernization Offices, Wudan 024500,China

Received:2010-10-25 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract

Abstract: According to MTNR1a cDNA sequence of sheep(gi:15U14109), human(gi:U14108), cattle(gi:U73327), mice(gi:U52222) and other species announced in GenBank,primers were designed. The 275 bp fragment of exon 1 of MTNR1a gene was amplified by PCR in Inner Mongolian Cashmere goat. The PCR products were cloned and sequenced. By compared with database of GenBank, it turned out that homology of nucleotide sequence of exon 1 MTNR1a gene in Cashmere goat with sheep, cow were 99% and 96%, respectively. It showed that the sequence was MTNR1a gene exon 1 sequence in Cashmere goat. Using DNAStar software to analysis, 257 nucleotides of the gene sequence was gotten.The sequence contained 5'UTR 23 nucleotides, translation initiation codon ATG and the N terminal 78 amino acid coding sequences.

Key words: Cashmere goat; MTNR1a gene; cloning; sequence analysis 

CLC Number:

Q785

SUN Yong-ming;ZHANG Yan-jun;LI Guo-hua;LIU Zhi-hong;WANG Zhi-ying;YU Xin-lei;ZHAO Yan-hong. Cloning and Sequence Analysis of Exon1 MTNR1a Gene of Inner Mongolian Cashmere Goat[J]. , 2011, 38(5): 82-84.

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Cloning and Sequence Analysis of Exon1 MTNR1a Gene of Inner Mongolian Cashmere Goat

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