›› 2011, Vol. 38 ›› Issue (5): 241-243.

• 经验交流 • Previous Articles     Next Articles

Establishment of Double-antibody Sandwich ELISA for Eperythrozoon ovis

ZHANG Fu-mei, HAN Fu-sheng, LI Ji-lian, LI Zi-ping   

  1. College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China
  • Received:2010-10-21 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract: To diagnose and detect Eperythrozoonosis on sheep quickly and take preventive measures in time, the double-antibody sandwich ELISA was established. The blood samples of sheep from large-scale farms whose red cell infective rate was over 90% were selected. Eperythrozoon ovis antigen was isolated, rabbit anti-sheep antibody was purified, antibody was labeled with HRP, then double-antibody sandwich ELISA was established. The results showed that the optimal working concentration of IgG was 82.91 μg/mL, while the best enzyme-conjugated antibody concentration was 1∶400, and the detective minimum of antigen was 7.81 μg/mL. The specific test and crossing reaction indicated that the double-antibody sandwich ELISA was a quick, sensitive way adapted to diagnosis and colony check.

Key words: sheep; Eperythrozoon; double-antibody sandwich ELISA

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