›› 2011, Vol. 38 ›› Issue (5): 237-240.

• 经验交流 • Previous Articles     Next Articles

Production and Improvement of Handmade Somatic Cell Cloning Tools

HUANG Zhang-hu, REN Yan-ping, TAN Shi-jian   

  1. Animal Reproduction Institute,Guangxi University,Nanning 530004,China
  • Received:2010-10-12 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract: Handmade somatic cell cloning(HMC) technology resolves the huge difficulty for the application of somatic cell nuclear transfer technology in the actual production which is the high equipment costs. It is not only save more costs by using self-made micro-knife,but also could fit user’s customary well,so it improves cutting efficiency. The results showed that the speed of enucleation was 496 oocytes per hour by cutting oocytes used self-made micro-knife and the recovered rate was 88.14%. Moreover,the feature of Well of Well needle which was easy to produce and could be polished.It was play a important role in cost saving and improving efficiency of HMC technology.

Key words: HMC; self-made micro-knife; Well of Well needle; HMC cutting method 

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