China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (3): 1207-1217.doi: 10.16431/j.cnki.1671-7236.2023.03.035

• Basic Veterinary Medicine • Previous Articles     Next Articles

Anti-MRV Activity of Acanthopanax senticosus Aqueous Extract (ASAE) and Its Immunomodulatory Effects on Micropterus salmoides

MA Yanping1, XU Jingjing2, YANG Hongwei1, HAO Le1, JIA Mengyan3, TAO Ziqian2, WANG Jingya4, HUANG Wen5, PENG Kai5, LIU Zhenxing1   

  1. 1. Collaborative Innovation Center of GDAAS, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    3. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
    4. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
    5. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
  • Received:2022-09-20 Online:2023-03-05 Published:2023-03-02

PDF (PC)

106

Abstract: 【Objective】 The aim of this study was to investigate the inhibitory effect of Acanthopanax senticosus aqueous extract (ASAE) on Mandarin ranavirus (MRV) and the immunomodulatory effects of Micropterus salmoides.【Method】 In this study,the inhibitory activity of ASAE on MRV in Siniperca chuatsi brain cell 3 lines (SCB3) was detected with different doses of ASAE (1.2,1.0,0.8,0.6,0.4 and 0.2 mg/mL) and MRV. 5‰ ASAE was added to the basal diet and fed Micropterus salmoides for 15 days,the phagocytosis effect of ASAE on Micropterus salmoides was analyzed by quantitative Real-time PCR and flow cytometry,and the protective effect of ASAE on MRV was determined by MRV infection test (1×107 TCID50/mL MRV,intraperitoneal injection).【Result】 The inhibition test results showed that MRV inhibition rates were 95.27%,91.36%,84.55%,60.05%,32.16% and 2.49% when the different concentrations of ASAE (1.2,1,0.8,0.6,0.4 and 0.2 mg/mL) were added into SCB3 cell lines.The quantitative Real-time PCR results showed that the ASAE induced significant up-regulation of cell phagocytosis pathway genes including interleukin-10 (IL-10),tyrosine-protein kinase (Lyn) and actin related protein 2/3 complex,subunit 5 (ARPC5), and Fcγ receptor Ⅰa (FcγR Ⅰa) in Micropterus salmoides (P＜0.05).The flow cytometry analysis showed that the ASAE significantly increased the phagocytosis ability of head kidney leukocytes of Micropterus salmoides (80.9% phagocytosis rate).The infection results showed that the relative protective rate of ASAE group was 30.48%.Compared with the control group,ASAE significantly delayed the process of infection and death of Micropterus salmoides. The virus copy numbers in brain,spleen,midgut and hindgut tissue of ASAE group were significantly decreased (P＜0.05).【Conclusion】 The results proved that ASAE significantly activated the phagocytic activity of Micropterus salmoides and played an excellent immunoregulatory role.Through the vitro and vivo tests,ASAE had excellent anti-MRV effect and could effectively prevent MRV infection.

Key words: Acanthopanax senticosus aqueous extract (ASAE); Mandarin ranavirus (MRV); Micropterus salmoides; in vitro test; in vivo test; phagocytic effect

CLC Number: