China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (3): 1140-1149.doi: 10.16431/j.cnki.1671-7236.2023.03.028

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution

ZHU Xihui1, LI Yanlan1, WANG Zhiwei1, PANG Zheyu1, LI Yubao1, LIU Cheng1, SI Zhenshu1, LU Jianbiao1, CAO Shengliang1, XUE Xijuan2   

  1. 1. College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng 252059, China;
    2. Shandong Xinde Technology Co., Ltd., Qingdao 266000, China
  • Received:2022-07-18 Online:2023-03-05 Published:2023-03-02

Abstract: 【Objective】 This study was aimed to understand the antibiotic resistance,genome structure and degree of genetic variation of Riemerella anatipestifer,so as to provide a reference for studying of antibiotic resistance and genome of Riemerella anatipestifer.【Method】 PCR identification,antibiotic resistance detection and draft whole genome sequencing of a suspected strain of Riemerella anatipestifer were performed,this suspected strain was isolated from brain tissue of died ducks,and its genetic evolutionary analysis was performed based on 16S rDNA and housekeeping genes.【Result】 After quality control and filtering,the sequencing data was successfully assembled into a draft genome containing 38 contigs.Six different antibiotic resistance genes were detected using Abricate software and CARD online database,four of which were consistent with the antibiotic resistance phenotype.The gene sequence was submitted to MLST database,and a new ST type (ST93) was found.The results of 16S rDNA and housekeeping gene phylogenetic trees showed that the isolates were closely related to two Riemerella anatipestifer strains.【Conclusion】 A strain of Riemerella anatipestifer was isolated and identified,which was a multiantibiotic resistant strain and carries multiple antibiotic resistance genes in its genome.The gene variation of Riemerella anatipestifer was large,and there was a risk of cross regional epidemic transmission.In clinical medication,we should pay attention to rational medication,timely monitor the horizontal transfer of resistance genes,and conduct regular epidemiological investigation to prevent the cross regional epidemic of Riemerella anatipestifer.

Key words: Riemerella anatipestifer; antibiotic resistance; antibiotic resistance gene; genome; bioinformatics

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