河北地区动物源大肠杆菌多重耐药性与流行性分析

doi:10.16431/j.cnki.1671-7236.2023.03.023

Abstract

Abstract: 【Objective】 This study was aimed to investigate the drug resistance of animal-derived Escherichia coli (E.coli) isolates in Hebei and the genetic relationship among the drug-resistant strains after the prohibition of resistance,so as to provide a scientific basis for the effective prevention and control of E.coli drug resistance.【Method】 297 fecal samples (48 from chickens and 249 from pigs) of healthy animals were collected from a farm in Hebei province.The isolates were identified by bacterial isolation and purification and PCR amplification,and the resistance of the isolates were determined by drug susceptibility test.Drug resistance gene detection,serotype identification,phylogenetic group analysis,plasmid replicon typing and genotyping were performed.【Result】 The isolates appeared as pink round colonies on MacConkey medium.A total of 129 strains of E.coli were isolated and identified by 16S rDNA sequencing analysis,of which 92 strains of E.coli showed multi-drug resistance,mainly 3-4 resistance,and the detection rate of the drug resistance genes were the highest with floR,tetA,qnrS and strB.The multidrug-resistant E.coli was dominated by O132 serotype,and the phylogenetic groups were mainly distributed in groups A and B1,and the main prevalent plasmids were IncFIB and IncN,27 ST types were obtained by MLST typing,mainly ST93 type,of which 12 sequence types were unknown type.【Conclusion】 The phenomenon of multi-drug resistance of E.coli in the intestine of healthy animals in Hebei was relatively serious,with numerous serotypes and diverse molecular characteristics,and it was still necessary to strengthen the monitoring of drug resistance and molecular characteristics of E.coli of animal origin.

Key words: Escherichia coli; drug resistance; molecular characteristics

CLC Number:

S852.61⁺2

ZHU Qiqi, ZHANG Jiyu, WANG Chengye, WU Lingyu, WANG Weiwei, ZHU Zhen. Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1081-1092.

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Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei

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