China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (3): 1037-1047.doi: 10.16431/j.cnki.1671-7236.2023.03.019

• Genetics and Breeding • Previous Articles     Next Articles

Polymorphisms of Four Polyembryony Related Genes and Its Association Analysis with Litter Size in Cashmere Goats

Sachula1, WU Tiecheng1, MA Yuejun1, HE Yunmei2, ZHU Lixian3, HE Tuoya4, WU Yuping5, LIU Bin1   

  1. 1. Inner Mongolia Academy of Agricultural and Husbandry Sciences, Hohhot 010031, China;
    2. Etuoke Agricultural and Animal Husbandry Technology Extension Center, Erdos 017000, China;
    3. Alashan Animal Husbandry and Veterinary Technology Promotion Center, Alashan 750030, China;
    4. Erdos Li Xin Industrial Corporation Limited, Erdos 017000, China;
    5. Hangjin Agricultural and Animal Husbandry Technology Extension Center, Erdos 017400, China
  • Revised:2022-11-28 Online:2023-03-05 Published:2023-03-02

Abstract: 【Objective】 Through sequencing and bioinformatics analysis of 4 polyembryony related genes in Inner Mongolia White cashmere goats,the single nucleotide polymorphisms (SNP) significantly related to litter size were deeply explored,so as to provide a theoretical basis for improving cashmere goat breeding efficiency.【Method】 244 cashmere goat ewes in type Alashan and Aerbasi were selected as candidates,and MultipSeq PCR combined with second-generation high-throughput sequencing technology was used to detect the polymorphism of growth differentiation factor 9 (GDF9),bone morphogenetic protein 15 (BMP15),bone morphogenetic protein receptor 1B (BMPR1B) and beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2) genes,the association between different SNPs and litter size of cashmere goats were analyzed.【Result】 A total of 172 SNPs were predicted by GATK analysis,including 95,33,26 and 18 SNPs related to BMPR1B,B4GALNT2,BMP15 and GDF9 genes,respectively.The correlation analysis results of GLM model showed that 10 SNPs were significantly correlated with the litter size (P<0.05),and among them genotypes of 7 SNPs illustrated significantly related with litter size with further chi-square test (P<0.05).The litter size of CC,GG,GA,CA and CC genotypes of BMPR1B gene at g.29893723 C>G,g.29897064 G>A,g.29897722 G>A,g.29897734 C>A and g.29938673 C>G were significantly higher than that of CG,GA,GG,CC and GG genotypes (P<0.05),respectively.The litter size of GG and GA genotypes of B4GALNT2 gene at g.37072289 G>A were significantly higher than that of AA genotype (P<0.05),and the litter size of CC and AC genotypes of GDF9 gene at g.66027842 A>C were significantly higher than that of AA genotype (P<0.05).【Conclusion】 The sequence characteristics of SNPs of GDF9, BMP5, BMPR1B and B4GALNT2 genes related to multiparity traits in Inner Mongolia White cashmere goats were obtained through the study,and a total of 10 SNPs were significantly related to litter size in cashmere goats,and revealed the difference among litter size of different genotypes.The results could provide a powerful reference SNP for molecular-assisted breeding of Inner Mongolia White cashmere goats.

Key words: cashmere goats; GDF9 gene; BMP15 gene; BMPR1B gene; B4GALNT2 gene; litter size

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