冷应激复温对ICR小鼠神经行为学的影响

doi:10.16431/j.cnki.1671-7236.2023.03.009

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of spontaneous movement,exploratory behavior,anxiety and blood hormone indexes in ICR mice with different cold stress rewarming.【Method】 Compared with 28 ℃ normal temperature control group,the movement changes of ICR mice in the open field test and elevated plus maze test after rewarming 2,4,6,8,10 and 12 h in 4,10,16 and 22 ℃ cold stress rewarming group were detected,respectively,and the changes of blood hormone indexes in each group after rewarming 2 and 4 h were detected by ELISA.【Result】 The results of open field test showed that compared with 28 ℃ normal temperature control group,the residence time and exercise time of central region were significantly reduced after 10 h of rewarming at 22 ℃ cold stimulation (P＜0.05).The residence time in the central region was significantly decreased after 4 and 8 h of rewarming at 16 ℃ cold stimulation (P＜0.05).The residence time in the central region was significantly decreased after 4 and 10 h of rewarming at 10 ℃ cold stimulation (P＜0.05),and the movement distance of the central region was significantly increased after 2 h of rewarming (P＜0.05).The residence time of the central region was extremely significantly decreased after 6 h of rewarming at 4 ℃ cold stimulation (P＜0.01),and the movement distance of the central region was extremely significantly increased after 2 h of rewarming (P＜0.01).Elevated plus maze test showed that compared with 28 ℃ normal temperature control group,the residence time of closed arms was significantly or extremely significantly decreased after 4 h of rewarming at 22 and 4 ℃ cold stimulation and 10 h of rewarming at 16 ℃ cold stimulation (P＜0.05 or P＜0.01).There was no significant change in the residence time of closed arm in 10 ℃ cold stimulation rewarming group (P＞0.05).Corticosterone (CORT) was extremely significantly increased after 4 h of rewarming at 22 ℃ cold stimulation (P＜0.01),and was extremely significantly increased after 2 and 4 h of rewarming at 16 ℃ cold stimulation (P＜0.01).Epinephrine (EPI) was extremely significantly increased after 4 h of rewarming at 22 ℃ cold stimulation (P＜0.01),and extremely significantly increased after 2 and 4 h of rewarming at 10 ℃cold stimulation (P＜0.01).【Conclusion】 After the end of cold stress,the spontaneous movement,exploration behavior,anxiety and blood hormone indexes of ICR mice would continue to be affected.It was necessary to reduce the temperature change of the test environment with ICR mice as the model and improve the accuracy of the test results.

Key words: ICR mice; cold stress; rewarming; roethology; corticosterone (CORT); epinephrine (EPI)

CLC Number:

S852.2

SHI Hongzhao, LIU Yang, YAO Ruizhi, LIU Peng, LI Shize. Effects of Cold Stress Rewarming on Neurobehavioral Changes in ICR Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 932-941.

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Effects of Cold Stress Rewarming on Neurobehavioral Changes in ICR Mice

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