miR-192进化分析、靶基因预测及组织表达分析

doi:10.16431/j.cnki.1671-7236.2023.03.004

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the biological evolutionary process and potential target gene function of miR-192,and the expression differences of miR-192 in adipose tissue of Daweizi pigs (obese-type) and Yorkshire (lean-type).【Method】 miR-192 mature sequences were obtained using the miBase database,and the distribution characteristics of miR-192 on the genomes of different species was located using the Ensembl database.The phylogenetic tree of miR-192 was constructed using the Mega 11.0. The expression of miR-192 in the dorsal adipose tissue of Daweizi pigs and Yorkshire were analysised by quantitative Real-time PCR,and the expression differences were compared.Multiple target gene prediction sites were used to predict the target genes that might bind to pig miR-192,and GO function and KEGG enrichment analysis were performed on the target genes.【Result】 miR-192 maintained a high degree of conservation during biological evolution.Quantitative Real-time PCR results showed that the expression of miR-192 in the dorsal adipose tissue of Daweizi pig was extremely significantly higher than that of Yorkshire (P<0.01). The results of GO function and KEGG pathway enrichment showed that the target gene of miR-192 mainly play the role of pre-transcriptional regulation and binding,and mainly function in chromatin and nucleus,and most genes were enriched in signaling pathways related to adipose metabolism.Five conserved target genes of pig miR-192 were obtained from the intersection of prediction results of different species on three websites.【Conclusion】 miR-192 was highly conserved in biological evolution and might regulate the expression of target genes by targeting,and played an important role in adipose tissue differentiation of pigs,which provided a theoretical basis for further research on the regulatory mechanism of miR-192 in adipose tissue differentiation.

Key words: pigs; miR-192; evolutionary analysis; adipose tissue; target gene

CLC Number:

CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.

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Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192

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