鸭产蛋量相关遗传变异和功能基因筛选及鉴定

doi:10.16431/j.cnki.1671-7236.2022.11.001

Abstract

Abstract: 【Objective】 The aim of this study was to compare single nucleotide polymorphism (SNP) and gene differences in regions with significant genomic differences between groups of ducks with different egg production using mixed pool whole-genome re-sequencing and selective clearance analysis technology, and screen and identify genetic variation sites and functional genes related to egg production, so as to provide a molecular basis for improving egg-laying performance.【Method】 In this study, two extreme phenotypes of Jinding ducks were selected according to individual egg production within 150 days after the opening:A high-egg-production group (CH) and a low-egg-production group (CL). SNP and related functional genes that located in regions with significant genomic differences between two groups were screened based on mixed pool whole-genome re-sequencing and selective clearance analysis technology.The screened SNP related to egg production were verified by PCR sequencing of each sample.GO function and KEGG pathway enrichment analysis were performed on the selected candidate genes to determine the most important biochemical metabolic pathway and signal transduction pathway involved by the candidate genes.And differences in egg production among different genotype of each SNP were analyzed.【Result】 192 071 438 and 229 836 820 clean reads were obtained in CL and CH groups, respectively.There were 1 368 significantly different SNP fragment regions, and 214 candidate genes were selected.These intervals and genes were mainly located on chromosome Z, mainly including KDM4C, LURAP1L, PTCH1, PRUNE2, TRPM3 and VPS13AD genes.The verification results showed that the re-sequencing results were accurate.GO function analysis showed that these selected genes were involved in three ontologies:Molecular function, cellular component and biological process.KEGG pathway enrichment analysis showed that these genes were mainly enriched in metabolic pathway, regulation of actin cytoskeleton, ribosome biogenesis in eukaryotes and other signal pathways.Multiple mutations, Z-28286537, Z-28286879, Z-28288421, Z-28434122, Z-28436368 in the candidate gene KDM4C, Z-30802227 in LURAP1L gene, Z-36500134, Z-36503668, Z-36534782, Z-36684262, Z-36710928, Z-36732487 in TRPM3 gene, Z-37498270, Z-37513004 in VPS13A gene, and Z-41510597 in PTCH1 gene were affected the ducks egg production.【Conclusion】 There were multiple SNPs and genes significantly related to egg production on chromosome Z in ducks. These results provided a molecular basis for improving egg-laying performance and accelerating the breeding process in ducks.

Key words: ducks; egg production; genetic variation; functional gene

CLC Number:

S813.1

TAO Zhiyun, ZHU Chunhong, LIU Hongxiang, SHI Zuhao, ZHANG Shuangjie, XU Wenjuan, SONG Weitao, WANG Zhicheng, LI Huifang. Screening and Identification of Genetic Variation and Functional Genes Related to Egg Production in Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4107-4118.

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Screening and Identification of Genetic Variation and Functional Genes Related to Egg Production in Ducks

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