水牛TRAIL基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2022.10.001

Abstract

Abstract: 【Objective】 The purpose of this study was to clone and analyze the CDS sequence of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) gene in buffalo,and its coding protein was analyzed by bioinformatics,so as to prepare for the study of effects of TRAIL protein on the development of follicle,proliferation and apoptosis of granulosa cells in buffalo ovary.【Method】 The CDS sequence of TRAIL gene in buffalo was cloned by RT-PCR.The similarity of nucleotide and amino acid sequences of TRAIL gene were analyzed,the phylogenetic tree was constructed,and the structure and function of TRAIL protein were analyzed by bioinformatics softwares.【Result】 The CDS sequence of TRAIL gene in buffalo was successfully cloned,with a length of 864 bp,encoding 287 amino acids.The similarity of TRAIL gene between buffalo and Bos mutus,Bos taurus,Sus scrofa, Capra hircus, Ovis aries,Equus caballus,Pan paniscus, Homo spiens,Mus musculus were 99.2%,99.3%,95.9%,96.3%,84.7%,84.8%,81.3%,81.3% and 70.0%,respectively.The phylogenetic tree results showed that buffalo had the closest genetic relationship with Bos mutus and Bos taurus,and the farthest genetic relationship with Mus musculus.Amino acid sequence alignment results showed that the transmembrane domain and TNF domain were highly conserved among different species.TRAIL protein was a hydrophilic protein and had a transmembrane domain,the 140-285 amino acids were TNF region.There was no glycosylation site and signal peptide of TRAIL protein,which were mainly located in the cytoplasm,and had 29 phosphorylation sites.The secondary structure of TRAIL protein was mainly random coil,accounting for about 51.57%,followed by extended chain (24.39%) and alpha helix (24.04%).The tertiary structure of TRAIL protein was consistent with the secondary structure,and the similarity with the model protein TRAIL protein in human was 75.53%.【Conclusion】 In this experiment,the CDS sequence of TRAIL gene was cloned in buffalo,with a size of 864 bp and encoding 287 amino acids.The buffalo had the closest relationship with Bos mutus and Bos taurus,and the TRAIL protein transmembrane domain and TNF domain were highly conserved among different species,which might be related to its function.

Key words: buffalo; TRAIL gene; cloning; bioinformatics analysis

CLC Number:

Q785

XU Yuanyuan, MO Xia, HUANG Chenqian, FENG Chao, LU Xingrong, MA Xiaoya, YANG Chunyan, ZHENG Haiying, SHANG Jianghua. Cloning and Bioinformatics Analysis of TRAIL Gene in Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3693-3703.

References

[1] CARSWELL E A,OLD L J,KASSEL R L,et al.An endotoxin-induced serum factor that causes necrosis of tumors[J].Proceedings of the National Academy of Sciences of the United States of America,1975,72(9):3666-3670.
[2] STELLER H.Mechanisms and genes of cellular suicide[J].Science,1995,267(5203):1445-1449.
[3] WILEY S R,SCHOOLEY K,SMOLAK P J,et al.Identification and characterization of a new member of the TNF family that induces apoptosis[J]. Immunity,1995,3(6):673-682.
[4] PITTI R M,MARSTERS S A,RUPPERT S,et al.Induction of apoptosis by Apo-2 Ligand,a new member of the tumor necrosis factor cytokine family[J].Journal of Biological Chemistry,1996,271(22):12687-12690.
[5] WATERS J P,POBER J S,BRADLEY J R.Tumour necrosis factor in infectious disease[J].Journal of Pathology,2013,230(2):132-147.
[6] GRUSS H J,DOWER S K.The TNF ligand superfamily and its relevance for human diseases[J].Cytokines and Molecular Therapy,1995,1(2):75-105.
[7] GOETZ F W,PLANAS J V,MACKENZIE S.Tumor necrosis factors[J]. Developmental & Comparative Immunology,2004,28(5):487-497.
[8] WARE C F.Protein therapeutics targeted at the TNF superfamily[J].Advances in Pharmacology,2013,66:51-80.
[9] DRR J,BECHMANN I,WAICZIES S,et al.Lack of tumor necrosis factor-related apoptosis-inducing ligand but presence of its receptors in the human brain[J].Journal of Neuroscience the Official Journal of the Society for Neuroscience,2002,22(4):RC209.
[10] LEE S H,SHIN M S,PARK W S,et al.Immunohistochemical analysis of Fas ligand expression in normal human tissues[J].Acta Pathologica,Microbiologica et Immunologica Scandinavica,1999,107(11):1013-1019.
[11] SPIERINGS D C.Tissue distribution of the death ligand TRAIL and its receptors[J].Journal of Histochemistry & Cytochemistry,2004,52(6):821-831.
[12] OUYANG X,SHI M,JIE F,et al.Phase Ⅲ study of dulanermin (recombinant human tumor necrosis factor-related apoptosis-inducing ligand/Apo2 ligand) combined with vinorelbine and cisplatin in patients with advanced non-small-cell lung cancer[J].Investigational New Drugs,2018,36(2):315-322.
[13] ROMAIN G,KEVIN B,GIULIA G,et al.In vitro and in vivo discrepancy in inducing apoptosis by mesenchymal stromal cells delivering membrane-bound tumor necrosis factor-related apoptosis inducing ligand in osteosarcoma pre-clinical models[J].Cytotherapy,2018,20(8):1037-1045.
[14] LIU Z,LI S,MA T,et al.Secreted TRAIL gene-modified adipose-derived stem cells exhibited potent tumor-suppressive effect in hepatocellular carcinoma cells[J].Immunity,Inflammation and Disease,2021,9(1):144-156.
[15] KIM H J,KANG S,KIM D Y,et al.Diallyl disulfide (DADS) boosts TRAIL-mediated apoptosis in colorectal cancer cells by inhibiting Bcl-2[J]. Food and Chemical Toxicology,2019,125:354-360.
[16] ARCIDIACONO M V,RIMONDI E,MAIETTI E,et al.Relationship between low levels of circulating TRAIL and atheromatosis progression in patients with chronic kidney disease[J].PLoS One,2018,13(9):e0203716.
[17] KONG X,LUO J,XU T,et al.Plumbagin enhances TRAIL-induced apoptosis of human leukemic Kasumi-1 cells through upregulation of TRAIL death receptor expression,activation of caspase-8 and inhibition of cFLIP[J].Oncology Reports,2017,37(6):3423.
[18] SONG T,ZHAND M,WU J,et al.Glioma progression is suppressed by Naringenin and APO2L combination therapy via the activation of apoptosis in vitro and in vivo[J].Investigational New Drugs,2020,38(6):1743-1754.
[19] ZHANG H,QIN G,YANG H,et al.TRAIL promotes epithelial-to-mesenchymal transition by inducing PD-L1 expression in esophageal squamous cell carcinomas[J].Journal of Experimental & Clinical Cancer Research:CR,2021,40(1):209.
[20] THISSE B,THISSE C.Functions and regulations of fibroblast growth factor signaling during embryonic development[J] Developmental Biology,2015,287(2):390-402.
[21] GOOD J,JOUBIN K,GIRALDEZ A J,et al.Nodal stability determines signaling range[J]. Current Biology,2005,15(1):31-36.
[22] WAND S B,XING B S,YI L,et al.Expression of Frizzled 2 in the mouse ovary during oestrous cycle[J].Journal of Animal Physiology and Animal Nutrition,2010,94(4):437-445.
[23] MANABE N,MATSUDA-MINEHATA F,GOTO Y,et al.Role of cell death ligand and receptor system on regulation of follicular atresia in pig ovaries[J]. Reproduction in Domestic Animals,2010,43(Suppl 2):268-272.
[24] 裴丽丽,章纬菁,卢佳,等.长江江豚TRAIL基因的克隆、体外表达及生物学功能分析[J].生物工程学报,2016,32(5):610-620. PEI L L,ZHANG W J,LU J,et al.Molecular cloning,in vitro expression and bioactivity of TRAIL gene from finless porpoises[J]. Chinese Journal of Biotechnology,2016,32(5):610-620.(in Chinese)
[25] 裴丽丽,侯欢欢,卢佳,等.蝙蝠TRAIL基因的克隆、表达及生物学功能分析[J].安徽农业科学,2015,43(22):83-86. PEI L L,HOU H H,LU J,et al.The cloning,expression and biological function of the bat TRAIL gene[J].Journal of Anhui Agricultural Science,2015,43(22):83-86.(in Chinese)
[26] MA L,SANG M,ZHANG J,et al.Identification,characterization and bioactivity of tumor necrosis factor (TNF)-related apoptosis-inducing ligand from Equus caballus[J].Developmental & Comparative Immunology,2016,67:340-349.
[27] SEOL D W,BILLIAR T R.Cysteine 230 modulates tumor necrosis factor-related apoptosis-inducing ligand activity[J].Cancer Research,2000,60(12):3152-3154.
[28] BODMER J L,MEIER P,TSCHOPP J,et al.Cysteine 230 is essential for the structure and activity of the cytotoxic ligand TRAIL[J]. Journal of Biological Chemistry,2000,275(27):20632-20637.
[29] GAO Y,CHANG M X,SUN B J,et al.TRAIL in the mandarin fish Siniperca chuatsi:Gene and its apoptotic effect in HeLa cells[J]. Fish & Shellfish Immunology,2008,24(1):55-66.
[30] KAHRAMAN S,YILMAZ O,ALTUNBAS H A,et al.TRAIL induces proliferation in rodent pancreatic beta cells via Akt activation[J].Journal of Molecular Endocrinology,2021,66(4):325-338.
[31] 刘鹏,李向臣,姚雅馨,等.卵巢颗粒细胞凋亡的调控因素[J].现代生物医学进展,2009,9(13):2562-2564. LIU P,LI X C,YAO Y X,et al.The regulating factors of ovary granular cell[J].Progress in Modern Biomedicine,2009,9(13):2562-2564.(in Chinese)
[32] LIU S,JIANG L,ZHONG T,et al.Effect of acrylamide on oocyte nuclear maturation and cumulus cells apoptosis in mouse in vitro[J].PLoS One,2015,10(8):e0135818.
[33] 刘秀兰,杨宏新,王妍,等.凋亡调控蛋白bcl-2、caspase-3在大鼠多囊卵巢颗粒细胞中的表达及生物学意义[J].内蒙古医科大学学报,2015,37(1):10-14. LIU X L,YANG H X,WANG Y,et al.The expression and biological significances of bcl-2 and caspase-3 apoptosis regulated protein in rats granulosa cells with PCOS[J].Journal of Inner Mongolia Medical University,2015,37(1):10-14.(in Chinese)
[34] WADA S,MANABE N,NAKAYAMA M,et al.TRAIL-decoy receptor 1 plays inhibitory role in apoptosis of granulosa cells from pig ovarian follicles[J].Journal of Veterinary Medical Science,2002,64(5):435-439.
[35] INOUE N,MANABE N,MATSUI T,et al.Roles of tumor necrosis factor-related apoptosis-inducing ligand signaling pathway in granulosa cell apoptosis during atresia in pig ovaries[J].The Journal of Reproduction and Development,2003,49(4):313-321.

Cloning and Bioinformatics Analysis of TRAIL Gene in Buffalo

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Yuanyuan, ZHENG Haiying, YANG Chunyan, DENG Tingxian, HUANG Chenqian, FENG Chao, LU Xingrong, DUAN Anqin, MO Xia, MA Xiaoya, SHANG Jianghua. Effects of Inhibition of BMP/Smad Signaling Pathway on the Growth and Steroid Hormone Synthesis of Buffalo Ovarian Granulosa Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2354-2362.
[2]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[3]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[4]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[5]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[6]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[7]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[8]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[9]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[10]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[11]	FU Yongzhi, MA Xiaojun, WANG Maohan, LI Zhe, CHEN Zhiyuan, QUAN Wei, ZHAO Han, SU Yongmei, WU Jianhua, ZHU Wenjin. Bioinformatics Analysis and Tissue Expression of MSTN Gene in Yangyuan Donkeys [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2910-2919.
[12]	QI Nannan, QIAN Yonghang, LUO Gang, SITU Xuqi, LIU Jiying. Cloning, Bioinformatics Analysis and Tissue Expression Location of Porcine MORC2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2431-2441.
[13]	ZHANG Hang, SHA Huiyang, LI Huawei, HUANG Liangzong, ZHAO Mengmeng. Cloning, Bioinformatics and Tissue Expression Analysis of TRIM3 Gene in Swine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2474-2483.
[14]	ZOU Jianwei, ZOU Juhong, SHEN Yujian, WEI Yi, ZHANG Sanbao, LIAN Zitong, XU Jianjian, SONG Ying, HUANG Yanna, WEI Yingming, JIANG Qinyang, ZHENG Zihua. Bioinformatics Analysis, Construction of Eukaryotic Expression Vector and Expression of LMCD1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2033-2042.
[15]	YAN Yelian, ZHANG Mengya, LIU Qiuchen, WANG Xin, XU Changzhi, ZONG Yanfeng, ZHU Zhihua, WU Sucheng, SONG Yu, LI Yunsheng, ZHANG Yunhai, CAO Zubing. Research Progress of Epigenetic Reprogramming in Mammalian Somatic Cell Nuclear Transfer Embryo Development [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2259-2269.