新型滴鼻灭活鹦鹉热衣原体疫苗的免疫效力评价

doi:10.16431/j.cnki.1671-7236.2022.05.036

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the effects of Vibrio cholerae ghosts (VCG) and chitosan gel (Gel) as compound adjuvants on enhancement of immunity of inactivated elementary body (EB) against Chlamydia psittaci infection.【Method】 Sixty SPF chickens aged 7 days were randomly divided into 4 groups,15 birds per group,including EB＋VCG＋Gel group,EB＋VCG group,Gel group and EB group.SPF chickens were immunized twice via nasal drop at an interval of 14 days.Specific IgG antibody levels,lymphocyte proliferation index,proportion of CD4^＋/CD8^＋ T cells,contents of cytokines (IL-4,IL-10,IL-12 and IFN-γ) were determined post immunization.Afterwards,throat bacteria excretion and lung lesions were detected post challenge with virulent Chlamydia psittaci strain.【Result】 Compared with Gel and EB groups,IgG antibody level,lymphocyte proliferation index and IFN-γ content and the ratio of CD4^＋/CD8^＋T cells in EB＋VCG＋Gel and EB＋VCG groups were significantly or extremely significantly increased (P<0.05;P<0.01).In addition,compared with Gel and EB groups,on the 12th day after challenge,the amount of laryngeal bacteria excretion in EB＋VCG＋Gel group was extremely significantly lower (P<0.01),on the 7th day after challenge,the lung lesion was extremely significantly lower (P<0.01).【Conclusion】 The novel nasal vaccine containing chitosan gel,VCG and inactivated EB was a promising approach by enhancing humoral and cellular responses,blocking transition via respiratory tracts,contributing to eradication the transmission via infected animals to human beings.

Key words: Chlamydia psittaci; nasal vaccine; immune efficacy; Vibrio cholera ghost (VCG); chitosan gel; chicken

CLC Number:

S852.67

CHEN Siyu, SUI Zhuojun, WANG Zhanxin, LI Qiang, CHEN Yi, WANG Fei, CHEN Shuang, LIU Xinyi, YAN Zhuanqiang, HE Cheng. Immune Efficacy of Novel Nasal Vaccine of Inactivated EB Against Chlamydia psittaci Challenge[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1951-1959.

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Immune Efficacy of Novel Nasal Vaccine of Inactivated EB Against Chlamydia psittaci Challenge

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