鸡肌内脂肪沉积相关候选基因筛选

doi:10.16431/j.cnki.1671-7236.2020.06.022

Abstract

Abstract: In this study,14 candidate genes related to the differential deposition of chicken intramuscular fat (IMF) screened by the research team in the transcriptome study of Jingxing-Huang chicken were performed for verification.To detect the association between the expression level of candidate genes and breast muscle IMF deposition in two breeds.The breast muscle of 98-day-old slow-growing local Jingxing-Huang chickens and 42-day-old fast-growing white feather Cobb broilers were used to distinguish the high and low phenotypic groups based on the content of triglycerides in the breast muscle.The results showed that there were significant differences in expression of 10 genes of ABCB8,ADIPOQ,BEND6,CD74,FKTN,HAT1,HS3ST5,MED4,TNFSF8 and TNIP1 between Jingxing-Huang chicken breast muscle TG high and low groups (P<0.05).There were significant differences in 7 genes of ADIPOQ,BEND6,FKTN,HAT1,HS3ST5,MED4 and TNIP1 between Cobb broiler breast muscle TG high and low groups (P<0.05).6 genes including ADIPOQ,FKTN,HAT1,HS3ST5,MED4 and TNIP1 were significantly expressed in both varieties (P<0.05).This study provided new candidate genes related to chicken IMF deposition,laying a good foundation for the study of IMF molecular regulation mechanism and related molecular marker screening research.

Key words: chicken; intramuscular fat; fat metabolism genes; Real-time quantitative PCR

CLC Number:

S831.2

LI Jinghui, XING Siyuan, WANG Xicai, LI Qinghe, ZHAO Guiping, ZHANG Yonghong, WEN Jie, LIU Ranran. Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1828-1836.

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Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens

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