猪流行性腹泻病毒反向遗传学及其研究进展

doi:10.16431/j.cnki.1671-7236.2020.06.007

Abstract

Abstract: Porcine epidemic diarrhea virus (PEDV) is the pathogen of porcine epidemic diarrhea (PED),a highly-contact intestinal infectious disease of pigs.PEDV is a single-stranded positive-stranded RNA virus with a cystic envelope,its genome is approximately 28 kb.Since 2010,the highly pathogenic PEDV G2 genotype strains have been continuously mutated,which has caused huge economic losses to the pig industry nationwide and even globally.Reverse genetic system is the full-length infectious clone of RNA virus construction.In recent years,PEDV is mainly based on the three methods of targeted RNA recombination,BAC system and in vitro connection to establish infectious cDNA cloning.The principle and method of reverse genetics were briefly summarized in this paper.Targeted RNA recombination utilizes the high homologous recombination of coronavirus RNA to achieve viral salvation,BAC system utilizes pBeloBAC11 vector to overcome the difficulty of cDNA instability in high-copy plasmid caused by the toxic sequence contained in PEDV genome.In vitro linkage technique mainly uses restriction endonuclease restriction sites existing in PEDV genome itself or modified restriction sites to connect virus fragments to full-length cDNA clones in vitro.In addition,this paper also summarized the recent progress in PEDV-related research based on reverse genetics technology.PEDV reverse genetics is an effective tool for studying the structure and function of PEDV virus genome and designing live attenuated vaccine,using reverse genetic technology to explore virulence related genes such as S gene,to explore the effect of mutations or deletions on the pathogenesis of the virus,to reveal the molecular mechanism of PEDV virulence attenuation,and to design live attenuated vaccine with good immunogenicity and avoid the return of virus and recombination of the virus strain.In conclusion,PEDV reverse genetics is an important method to study the genomic structure and function of PEDV,viral host interaction and pathogenic mechanism,as well as a reasonable and effective way to design a live attenuated PEDV vaccine.

Key words: porcine epidemic diarrhea virus (PEDV); reverse genetics; S gene; ORF3 gene; PEDV vaccine

CLC Number:

S852.65

LU Ying, SU Xueli, DU Chen, WANG Ruomu, ZHONG Lian, CHEN Ying, WEI Zuzhang, HUANG Weijian, OUYANG Kang. Reverse Genetics and Its Research Progress of Porcine Epidemic Diarrhea Virus[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1694-1701.

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Reverse Genetics and Its Research Progress of Porcine Epidemic Diarrhea Virus

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