China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (4): 1274-1281.doi: 10.16431/j.cnki.1671-7236.2020.04.035

• Clinical Veterinary Medicine • Previous Articles     Next Articles

Effect of Apoptosis Protein after Eimeria tenella Infection in Chicken

WANG Lixia1, SONG Licong2, ZHANG Jianjun2, AN Jian2   

  1. 1. College of Animal Science and Veterinary, Beijing Vocational College of Agriculture, Beijing 102442, China;
    2. College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China
  • Received:2019-11-06 Online:2020-04-20 Published:2020-04-17

Abstract: In order to study the effect of Eimeria tenella infection on apoptosis related proteins in chicken serum,and explore the interaction between Eimeria tenella and its host.240 7-day-old Heilan Grey male chickens were divided into 4 groups,group A was non infection and non induction of apoptosis,group B was non infection and induction of apoptosis,group C was infection and non induction of apoptosis,group D was infection and induction of apoptosis.The treatment method of inducing apoptosis was to take 40% alcohol to induce apoptosis at 6.4 mL/kg body weight at 2.5 h before blood collection,while the treatment of not inducing apoptosis was replaced by 40% alcohol and other volumes of normal saline.At 24,48,72,96 and 120 h after Eimeria tenella infection (1×105 Eimeria tenella sporulated oocysts per chicken),10 chickens in each group were collected for blood collection.The concentrations of Cytochrome C (Cyto-C),Caspase (Cas)-9,Cas-8,Cas-3,Bcl-2,Bax,Bcl-XL,Bid were detected by ELISA.The results showed that the concentrations of Cyto-C,Cas-9,Cas-8 and Cas-3 in group D were lower than those in group B at 24,48,72 and 96 h after infection.The concentrations of Cyto-C,Cas-9,Cas-8 and Cas-3 in group D at 120 h were significant higher than those in group B (P<0.05).The value of Bcl-2/Bax and Bcl-XL/Bid in group D at 24,48,72 and 96 h after infection were higher than those in group B.The values of Bcl-2/Bax in group D at 120 h were lower than that in group B and the value of Bcl-XL/Bid in group D was higher than that in group B.The results showed that the apoptosis of host cells was inhibited by the increase of Bcl-2/Bax value,Cyto-C,Cas-9,Cas-8 and Cas-3 at the early stage of Eimeria tenella infection,and was promoted by the decrease of Bcl-2/Bax value,Cyto-C,Cas-9,Cas-8 and Cas-3 at the middle and late stage of infection.

Key words: Eimeria tenella; chicken; cell apoptosis; protein

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