China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (4): 1058-1069.doi: 10.16431/j.cnki.1671-7236.2020.04.011

• Animal Nutrition and Feed Science • Previous Articles     Next Articles

Effects of Mixed Lipid on Breast Muscle Fatty Acid Composition, and Underlying Metabolomics Mechanism in Qingyuan Chickens

GOU Zhongyong, CUI Xiaoyan, FAN Qiuli, LI Long, LIN Xiajing, WANG Yibing, JIANG Shouqun, JIANG Zongyong   

  1. State Key Laboratory of Livestock and Poultry Breeding, The Key Laboratory of Animal Nutrition and Feed Science(South China), Ministry of Agriculture and Rural Affairs, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
  • Received:2019-09-09 Online:2020-04-20 Published:2020-04-17

Abstract: This study was conducted to examine the effects of dietary addition of soybean oil or a blend of oil and fat on the growth performance and fatty acid profile of breast muscle in chickens,and to explore the mechanism of metabolic changes occurring in the fatty acid profile of breast muscle based on metabolomics technology,which could provide new ideas for regulating the fatty acid composition of chickens.A total of 480 one-day-old female chicks (Qingyuan) were randomly assigned into two dietary treatments,each consisted of six replicates and 40 chicks per replicate.The diet of control group (CON) was added with soybean oil,and the experimental group (BOF) was added with the blend of oil and fat,soybean oil∶lard∶fish oil∶coconut oil=1.0∶1.0∶0.5∶0.5.The experimental period was 127 d.The results showed that the birds fed the BOF diet,compared to the controls,had similar daily body weight gain (P > 0.05),lower daily feed intake and feed∶gain ratio (P < 0.05);higher content of lauric acid (C12∶0),myristic acid (C14∶0),oleic acid (C18∶1n-9),eicosapentaenoic acid (EPA,C20∶5n-3) and docosahexaenoic acid (DHA,C22∶6n-3) (P < 0.05),and lower content of linoleic acid (C18∶2n-6) (P < 0.05) in breast muscle tissue.A total of 110 different metabolites (VIP > 1,P < 0.05) in the breast muscle were detected by liquid chromatography-mass spectrometry(LC-MS),due to the dietary treatment,63 of which were annotated and were mainly phospholipid metabolites,including phosphatidylcholine (PC),phosphatidylserine (PS) and phosphatidylethanolamine (PE).In addition,the contents of glutathione and carnosine in breast muscle were significantly increased in the BOF treatment (P < 0.05).Through the metabolic pathway enrichment analysis,it was found that fatty acid metabolism pathways,including alpha-linolenic acid metabolism,arachidonic acid metabolism,linoleic acid metabolism and glycerophospholipid metabolism were changed significantly (P < 0.05).In summary,the BOF diet improved the feed efficiency of chickens and promoted DHA and EPA deposition in the breast muscle compared to those birds fed traditional soybean oil diet.It had been proven that the dietary fat intervention modified the phospholipid metabolites,oxidative stability metabolites and lipid metabolism pathways in breast muscle.The identified differential metabolites and differential metabolic pathways could provide references for the future studies on regulating fatty acid composition in chicken’s breast muscle.

Key words: Qingyuan chickens; blend of oil and fat; fatty acid composition; muscle metabolomics; phospholipid metabolites

CLC Number: