鸡痘病毒FJ01株的分离鉴定

doi:10.16431/j.cnki.1671-7236.2020.02.029

Abstract

Abstract: To investigate the cause of death of diseased chickens in large-scale chicken farm in Fujian province,the diseased chickens with a large number of nodular scab in the crown and head skin were identified by PCR.After being identified as FWPV strain,the virus was isolated by inoculating chicken chorioallantoic membrane(CAM).The virus was subcultured with primary chicken embryo fibroblasts (CEF) and passage cells DF-1 cells,and the similarities and differences of the culture characteristics of the isolated strain on the two kinds of cells were observed.The distribution of the virus and the morphology of the virus particles were observed by ultra-thin sections of the CEF infected with the isolated strain.The homologies of TK and FPV175 genes were analyzed.The results showed that the CAM inoculated with antibiotics showed a large area of single white protuberant acne spots,and the CEF and DF-1 cells were inoculated at the same time.The two kinds of cells could produce stable and sustainable passage cytopathic effect,but the time and degree of the lesion were different.Under the transmission electron microscope,the typical fowlpox virus particles were densely distributed in the cytoplasm of the infected CEF,and the sunken cores wrapped by the oval outer membrane were clearly seen.According to the statistics of 23 virion particles,the size of virion particles was (258 to 344) nm×(153 to 238) nm.PCR detection of FWPV TK gene and FPV175 gene showed that those nucleotide sequences of FWPV (accession No.:NC_002188.1) collected by GenBank were 100% and 99.8% homology,respectively.The above results indicated that the isolated strain was FWPV and was named as FWPV-FJ01,which provided a reference for the prevention and control of domestic FWPV.

Key words: fowlpox virus (FWPV); isolation and identification; virion; Fujian isolate FJ01

CLC Number:

S858.31

GAO Yingxue, LIN Min, HU Huanyi, LIU Xiaolan, LIU Changjin, WAN Wenzhong, LUO Feng, DENG Shunzhou. A Isolation and Identification of Fowlpox Virus FJ01 Strain[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 575-582.

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A Isolation and Identification of Fowlpox Virus FJ01 Strain

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