China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2778-2786.doi: 10.16431/j.cnki.1671-7236.2019.09.034

• Basic Veterinary Medicine • Previous Articles     Next Articles

Study on the Metabolism of Artemisinin in Chickens by LC-MS/MS

HAN Yiqing1,2, JI Xun1,2, CHEN Chunqin1,2, LIU Mingjiang1,2, LI Jingui1,2   

  1. 1. College of Veterinary Medicine, Yangzhou University, Yanghzou 225009, China;
    2. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
  • Received:2019-02-23 Online:2019-09-20 Published:2019-09-21

Abstract: In order to study the metabolism of artemisinin (ART) in chickens,the LC-MS/MS method was established for the determination of ART in chicken plasm.The electrospray ionization (ESI) source was used as the ion source,and artemether (ARM) was the internal standard(IS).The[M+Na]+ quasi ion peaks of ART and ARM were chosed for secondary mass spectrometry.Two pairs of ions,m/z 305.2→151.3 (ART) and m/z 321.0→163.1 (ARM),were selected for quantitative analysis by multi-monitoring reaction (MRM).It was concluded that there was a good linear relationship in the range of 10-1 000 ng/mL (R2=0.9997).In this method,the minimum quantitative limit was 10 ng/mL and the recovery rate was between 90% and 105%.Meanwhile,the intra-day and inter-day precision (RSD) were less than 15%.Thirty-day-old chickens were divided into six groups randomly according to the three doses (15,40 and 100 mg/kg) and the number of oral times (one or eleven).The one-time groups were administered ART at the 11th time,and the solvent was orally administered 10 times before.Eleven-time groups were fed ART for 11 times.After the last oral medication,300 μL blood of each chicken was collected in anticoagulant tube at 10 min,30 min,1 h,1.5 h,2 h,2.5 h,3 h,4 h,6 h and 8 h.The results showed that the concentration of ART in the blood of the chicken which were administered for one time (15,40 and 100 mg/kg) could reach the Cmax at 1 or 1.5 h.And the metabolism of ART in chickens could be accelerated by orally administered for more times and has a dose-dependent manner.In conclusion,the method established in this study was simple,sensitive and reproducible for the determination of ART in chicken plasma.There was a self-induced metabolism of ART in chickens,meanwhile,higher the dose,faster the metabolism.

Key words: LC-MS/MS; chickens; artemisinin; pharmacokinetics; autoinduction

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