China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2760-2768.doi: 10.16431/j.cnki.1671-7236.2019.09.032

• Basic Veterinary Medicine • Previous Articles     Next Articles

Identification and Susceptibility Test of Pathogenic Aeromonas veronii Isolated from Mauremys sinensis

FANG Zhenhua1, ZENG Yumei2, HONG Meiling2, DING Li2   

  1. 1. School of Tropical Agricultural Technology, Hainan College of Vocation and Technique, Haikou 570216, China;
    2. Key Laboratory for Ecology of Tropical Islands, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou 571158, China
  • Received:2019-03-14 Online:2019-09-20 Published:2019-09-21

Abstract: In order to identify the pathogen of a serious disease occurred in Mauremys sinensis,a dominant bacteria was isolated from the diseased Mauremys sinensis.It was identified by bacterial isolation and culture,Gram staining microscopy,biochemical test,16S rRNA sequence analysis,animal regression test and drug susceptibility.The isolate was gray-white,opaque,round,neat edge,and had wet and smooth surface with middle protruding colonies in general nutrient agar medium,and orange-red,round,smooth colonies in SS agar medium.Gram staining microscopy showed that the isolate was Gram-negative,short rod-shaped,obtuse round at both ends of the body,single or double-existent.Biochemical tests showed that the isolate was mobilized with positive reactions such as glucose,sucrose,galactose,maltose,fructose,arginine dihydrolase,ornithine decarboxylase,D-glucose acid production,D-glucose gas production,gelatin hydrolysis,oxidase,indole and V-P test.The sequence analysis of 16S rRNA showed that the isolate was clustered with Aeromonas veronii registered in GenBank,and had 99% homology with that reference strains.The animal regression test showed that HNJ002 was highly pathogenic to Mauremys sinensis.Drug sensitivity test showed that the isolate was sensitive to most antibacterial,moderately sensitive to roxithromycin,tobramycin and neomycin,and only resistant to a few antibiotics such as ampicillin,penicillin and clindamycin.This study would provide a scientific basis for the prevention and treatment of Aeromonas veronii in Mauremys sinensis.

Key words: Aeromonas veronii; Mauremys sinensis; isolation and identification; antibiotic sensitivity

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