中药牛皮消多糖体外抗新城疫病毒作用的研究

doi:10.16431/j.cnki.1671-7236.2019.09.029

Abstract

Abstract: In order to investigate the effect of Cynanchum auriculatum polysaccharide (CAP) on the resistance to Newcastle disease virus (NDV) in vitro,the polysaccharides from Cynanchum auriculatum was extracted by water and alcohol precipitation,phenol sulfuric acid and infrared spectroscopy were used to detect CAP.The safe concentration of CAP on chicken embryo fibroblast (CEF) was determined by MTT method,the safe concentration of CAP was set up to 625 μg/mL,and 5 concentrations in the safe concentration range (39.06 to 625 μg/mL) were selected.Each CAP and NDV were added with three kinds of dosing methods (add CAP and then inoculate the virus,inoculate the virus and then add CAP,CAP and the virus-sensing) to detect the blocking,inhibition and direct effect of CAP on NDV.The results showed that CAP₅₀,CAP₃₀,CAP_t,CAP₈₀ and CAP₇₀ could significantly inhibit the ability of NDV to infect CEF after the virus was first added group and the CAP and the virus-sensing group.The inhibition rates of CAP₅₀,CAP₃₀,CAP_t,CAP₈₀ and CAP₇₀ were 131.23%,110.12%,108.15%,100.24%,93.07% and 61.76%;43.73%,44.51%,29.02% and 37.45%,respectively.The addition of CAP₅₀ and CAP₃₀ in CAP followed by the virus group could significantly inhibited NDV (P<0.05) with 15.82% and 8.33% inhibition rate under the blocking condition,and the other groups had no inhibitory effect on NDV within the safe concentration range.In conclusion,the CAP had better anti-NDV activity,and the inhibition and direct killing effect on NDV was stronger than the blocking effect.CAP₅₀ and CAP₃₀ had stronger anti-NDV activity and could be used as research materials to study further.

Key words: Cynanchum auriculatum polysaccharide; chicken embryo fibroblast (CEF); Newcastle disease virus (NDV); antivirus

CLC Number:

S854.5

BUHELIQIANMU·Yiming, SAIFUDING·Abula, AIKEBAIER·Yiming, HUANGULI·Nuerbieke, AKEZHULI·Nuerbao, LI Hao. Study on Antiviral Effect of Polysaccharides from Chinese Medicine Cynanchum auriculatum on Newcastle Disease Virus in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2737-2744.

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Study on Antiviral Effect of Polysaccharides from Chinese Medicine Cynanchum auriculatum on Newcastle Disease Virus in vitro

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