国内猪源睾丸酮丛毛单胞菌的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2019.08.032

Abstract

Abstract:

In order to ascertain the cause of the stillbirth and weak piglets of sows in a large-scale pig farm in Fuyuan county, Qujing city, Yunnan province in August 2018, in this experiment, weak piglets visceral tissue materials were collected for isolation, culture, biochemical identification, 16S rRNA amplification, drug sensitivity test and pathogenicity test.The results showed that a small, translucent Gram-negative brevibacterium was isolated and cultured from weak piglets brain tissue.Biochemical identification results showed that itaconate assimilation (ITA), suberic acid salt assimilation (SUB), acetate (ACE), DL-lactate assimilation (LAT), propionate assimilation (PROP), 3-Hydroxy-butyrate (3OB), proline (PRO) biochemical reaction results were positive;Alanine assimilation (ALA), D-mannitol (MAN), D-glucose (GLU), citrate (CAP), serine assimilation (SER) and other biochemical reactions were negative.The 16S rRNA sequence had 99% homology with the 16S rRNA nucleotide sequence of Comamonas sp.EB172 strain (accession No.:EU847238.1) in GenBank.The susceptibility test showed that it was sensitive to amoxicillin/clavulanic acid, cefotaxime, polymyxin E, cotrimoxazole, spectinomycin, nitrofurantoin and doxycycline.The pathogenicity test showed that mice were inoculated intraperitoneally with 0.3 mL of 9.3×10⁸ CFU/mL bacterial suspension, and 7 mice died within 7 h (7/12).The results of this test indicated that there was a testicular infection of C.testosteroni in weak piglets and aborted fetuses.

Key words: porcine Comamonas testosteroni; isolation; identification

CLC Number:

S852.61

ZHAN Jianju, ZHANG Sheng, ZHU Laiping, MA Fen, YAO Jun, GAO Lin, WANG Shengkui. Isolation and Identification of Comamonas testosteroni from Porcine in China[J]. , 2019, 46(8): 2455-2461.

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Isolation and Identification of Comamonas testosteroni from Porcine in China

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