鲤源嗜水气单胞菌分离鉴定、耐药性及毒力基因检测

doi:10.16431/j.cnki.1671-7236.2019.07.035

Abstract

Abstract:

In order to find out the cause of death of carp in a carp farm in Guizhou,this experiment carried out bacterial isolation and culture,morphological observation of bacteria,physiological and biochemical identification of bacteria,16S rRNA gene sequencing,bacterial drug sensitivity test,drug resistance gene detection,animal infection test,virulence gene detection and so on.The results showed that the bacteria isolated from the diseased tissues of dead carp were round,gray-white colony with smooth surface and were Gram-negative brevibacterium.Biochemical identification showed that the isolated bacteria were motile,could ferment glucose to produce acid and gas,and were positive for arginine dihydrolase,lysine decarboxylase,M-R and V-P tests.The bacterial 16S rRNA gene universal primers were used for PCR amplification,and obtained a 1 421 bp fragment.Sequencing results showed that the isolate had 75.3% to 100.0% homology with 16 strains of Aeromonas hydrophila.Drug susceptibility test results showed that the isolate was highly susceptible to florfenicol,ceftriaxone,tobramycin,ofloxacin and enrofloxacin,and was resistant to compound sulfamethoxazole,sulfamethoxazole and carbenicillin.PCR result showed that the isolate carried Sul1,Sul2 and Intl1 resistance genes,which were consistent with the drug sensitivity phenotype.Artificial infection test showed that the isolate was pathogenic.Virulence gene test results showed that the isolate carried aer,hly and act 3 virulence genes.In conclusion,a sulfonamide-resistant strain of Aeromonas hydrophila from carp was isolated in this study,which provided a scientific basis for disease control and rational drugs use for carp in this farm.

Key words: carp; Aeromonas hydrophila; isolation and identification

CLC Number:

S917.1

HU Andong, ZHANG Mingyang, ZHANG Piao, YANG Xia, CHENG Zhentao, JIANG Haibo, WEN Ming. Isolation, Identification,Drug Resistance and Virulence Genes Detection of Aeromonas hydrophila from Carp[J]. , 2019, 46(7): 2167-2175.

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Isolation, Identification,Drug Resistance and Virulence Genes Detection of Aeromonas hydrophila from Carp

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