屠宰生猪副猪嗜血杆菌的分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2019.07.033

Abstract

Abstract:

In order to investigate the hidden infection of Haemophilus parasuis (Hps) in slaughtered pigs,this study collected 80 diseased lungs from a large-scale pig slaughterhouse in Shaanxi province.The positive lungs were aseptically sampled,and the suspected Hps was obtained by streaking through the plate.The morphology of the isolates,analysis of the culture characteristics,observation of the satellite phenomenon,biochemical test and sequence analysis of the isolates nucleic acid fragments were carried out,and the Hps isolates were purified.The sensitivity of Hps isolates to commonly used drugs was analyzed by drug sensitive paper method.The results showed that 39 Hps positive samples were detected from 80 lungs,and the positive rate was 48.75% (39/80).Three suspected Hps were isolated from 39 Hps-positive lungs.The isolate was Gram-negative,short rod under the microscope,grew well on TSA plates supplemented with fetal bovine serum and NAD,exhibited a typical "satellite phenomenon" growth around S.aureus,could ferment glucose,sucrose,fructose,galactose,maltose and arabinose without fermenting mannitol.The PCR amplification sequence was more than 98% homologous to Hps.The results of drug susceptibility test showed that the three isolates were highly sensitive to antibacterial drugs such as ciprofloxacin,enrofloxacin and florfenicol,and were not sensitive to antibacterial drugs such as penicillin,lincomycin and trimethoprim.The Hps isolates obtained in this study provided the basic materials for vaccine development,and provided a reference for the selection of sensitive drugs to prevent and control Hps infection in pig farms.

Key words: Haemophilus parasuis(Hps); isolation and identification; drug sensitivity test

CLC Number:

S852.61

TIAN Haolun, FENG Xiu, WANG Kai, FENG Quanwen, LI Xinxin, FAN Zhixin, LUO Lihua, SUN Ying, ZHOU Hongchao, ZHANG Yanming, GUO Kangkang. Isolation, Identification and Drug Sensitivity Analysis of Haemophilus parasuis in Slaughter Pigs[J]. , 2019, 46(7): 2151-2158.

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Isolation, Identification and Drug Sensitivity Analysis of Haemophilus parasuis in Slaughter Pigs

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