血清4型禽腺病毒W株的分离鉴定和全基因组序列分析

doi:10.16431/j.cnki.1671-7236.2019.07.022

Abstract

Abstract:

In order to understand the complete gene sequence,structural characteristics and genetic variation of fowl adenovirus genotype 4 (FAdV-4) W strain,the liver and kidney of infected chicken were identified by PCR.The isolation of SPF chicken embryo and electron microscope observation showed that the virus was FAdV-4 and TCID₅₀ was 10^-7.2/0.1 mL.The virus was about 70 nm in diameter and without envelope under electron microscope.Chorioallantoic membrane inoculation of 10-day-old SPF chicken embryos could significantly inhibit the development of chicken embryos and produce dwarf embryos,and the death rate was 100% within 10 days after inoculation with chorioallantoic membrane.The complete genome of W strain was amplified and sequenced.The virus was 43 591 bp,which had 60 open reading frames.The nucleotide sequence homology was 98.4% to 100% between W strain and referenced FAdV-4 strains published in GenBank.The homology was 98.4% with standard non-pathogenic virus strain ON1 (accession No.:GU188428.1).The ORF19 (lipase gene) and ORF27 were not found.It was found that W strain was closely related to the domestic isolates by analyzing the Hexon,Fiber-1 and Fiber-2 genes of the main structural proteins in recent years.It had a great difference with the ON1 strain and other foreign strains.The results showed that the strain had high pathogenicity and was different from foreign isolates,which provided the basis data for further analysis of the virulence enhancement mechanism of FAdV-4.

Key words: fowl adenovirus; isolation; identification; Fiber gene

CLC Number:

S852.65⁺9.1

ZHAO Yujie, LIU Jianxun, CHEN Tiantian, WANG Sainan, HE Chunhui, CHEN Panpan, LIU Lin, LI Xinsheng. Isolation, Identification and Complete Genome Sequence Analysis of Fowl Adenovirus Genotype 4 W Strain[J]. , 2019, 46(7): 2059-2068.

References

[1] ASTHANA M,CHANDRA R,KUMAR R.Hydropericardium syndrome:Current state and future developments[J]. Archives of Virology,2013,158(5):921-931.
[2] HESS M.Detection and differentiation of avian adenoviruses:A review[J]. Avian Pathology,2000,29(3):195-206.
[3] 汪镇南,唐青海,颜爱,等.猪腺病毒3型Hexon蛋白多克隆抗体制备与免疫活性鉴定[J].中国畜牧兽医,2015,42(1):1-8. WANG Z N,TANG Q H,YAN A,et al.Preparation and immuno-activity identification of polyclonal antibodies against porcine adenovirus serotype 3 hexon protein[J]. China Animal Husbandry & Veterinary Medicine,2015,42(1):1-8.(in Chinese)
[4] 刘雪美,秦建如,李育豪,等.Ⅱ群禽腺病毒五邻体蛋白单因子血清的制备[J].中国畜牧兽医,2013,40(10):16-20. LIU X M,QIN J R,LI Y H,et al.Preparation of uni-factor serum against penton protein of group Ⅱ avian adenovirus[J]. China Animal Husbandry & Veterinary Medicine,2013,40(10):16-20.(in Chinese)
[5] PRING P,ADRIAN T.Characterization of adenovirus subgenus D fiber genes[J]. Virology,1995,206(1):564-571.
[6] MEI Y F,WADELL G.Highly heterogeneous fiber genes in the two closely related adenovirus genome types Ad35p and Ad34a[J]. Virology,1995,206(1):686-689.
[7] GAHERY H,FARACE F,GODFRIN D,et al.Immune response to recombinant capsid proteins of adenovirus in humans:Anti-fiber and anti-penton base antibodies have a synergistic effect on neutralizing activity[J]. Virology,1998,72(3):2388-2397.
[8] BRADLEY R R,LYNCH D M,IAMPIETRO M J,et al.Adenovirus serotype 5 neutralizing antibodies target both hexon and fiber following vaccination and natural infection[J]. Virology,2012,86(1):625-629.
[9] YU B,DONG J,WANG C,et al.Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera[J]. Virology,2013,437(2):118-123.
[10] 殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997. YIN Z,LIU J H.Animal Virology[M].2nd Edition.Beijing:Science Press,1997.(in Chinese)
[11] BALAMURUGAN V,KATARIA J M.The hydropericardium syndrome in poultrya current scenario[J]. Veterinary Research Communication,2004,28(2):127-148.
[12] DOMANSKA-BLICHARZ K,TOMCZYK G,SMIETANKA K,et al.Molecular characterize ation of fowl adenoviruses isolated from chickens with gizzard erosions[J]. Poultry Science,2011,90(5):983-989.
[13] 陈亮,黄路生,刘晓文,等.鸭2型腺病毒的分离鉴定及 hexon 基因的序列分析[J].中国兽医科学,2016,46(12):1538-1542. CHEN L,HUANG L S,LIU X W,et al.Isolation and identification of duck adenovirus 2 and sequence analysis of hexon gene[J]. Chinese Veterinary Science,2016,46(12):1538-1542.(in Chinese)
[14] 王颖,任国鑫,金鑫,等.2株Ⅰ群禽腺病毒的分离鉴定及致病性分析[J].中国畜牧兽医,2018,45(12):3563-3571. WANG Y,REN G X,JIN X,et al.Isolation,identification and pathogenic analysis of two strains fowl adenovirus Ⅰ[J]. China Animal Husbandry & Veterinary Medicine,2018,45(12):3563-3571.(in Chinese)
[15] PAN Q,LIU L,GAO Y,et al.Characterization of a hypervirulent fowl adenovirus 4 with the novel genotype newly prevalent in China and establishment of reproduction infection model of hydropericardium syndrome in chickens[J]. Poultry Science,2017,96(6):1581-1588.
[16] YE J,LIANG G,ZHANG J,et al.Outbreaks of serotype 4 fowl adenovirus with novel genotype,China[J]. Emerging Microbes & Infections,2016,25(5):e50.
[17] 卡尔尼尔主编.高福,苏敬良译.禽病学[M].第10版.北京:中国农业出版社,2012. CALNEK B W (editor).GAO F,SU J L (translator).Disease of Poultry[M].10th Edition.Beijing:China Agriculture Press,2012.(in Chinese)
[18] DINESHN M,NARESH J,ASHOK K T,et al.Characterization of fowl adenovirus associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens[J]. Virus Disease,2014,25(1):114-119.
[19] MACLACHLAN N,DUBOVI E J主编.孔宪刚,刘胜旺译.兽医病毒学[M].北京:中国农业出版社,2015. MACLACHLAN N,DUBOVI E J (editor).KONG X G,LIU S W (translator).Fenner's Veterinary Virology[M].Beijing:China Agriculture Press,2015.(in Chinese)
[20] VERA P F,MORALES A,CORTES D V,et al.Clinicopathological characterization and genomic sequence differences observed in a highly virulent fowl aviadenovirus serotype 4[J]. Avian Pathology,2016,45(1):73-81.
[21] 王娟,刘亮亮,李慧昕,等.禽腺病毒血清4型的分离鉴定及遗传演化分析[J].中国兽医科学,2017,47(6):755-761. WANG J,LIU L L,LI H X,et al.Isolation,identification and phylogenetic analysis of fowl adenovirus serotype 4[J]. Chinese Veterinary Science,2017,47(6):755-761.(in Chinese)
[22] MASE M,NAKARMURA K,IMADA T.Characterization of fowl adenovirus serotype 4 isolated from chickens with hydropericardium syndrome based on analysis of the short fiber protein gene[J]. Journal of Veterinary Diagnostic Investigation,2010,22:218-223.
[23] 罗洋洋,招丽婵,李群辉,等.Ⅰ群禽腺病毒血清4型广东株的分离鉴定及分子特性[J].华南农业大学学报,2017,38(6):27-33. LUO Y Y,ZHAO L C,LI Q H,et al.Isolation,identification and molecular characteristics of group Ⅰ fowl adenovirus serotype 4 from Guangdong,China[J]. Journal of South China Agricultural University,2017,38(6):27-33.(in Chinese)

Isolation, Identification and Complete Genome Sequence Analysis of Fowl Adenovirus Genotype 4 W Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	PENG Yangxiang, YANG Zhenbiao, YAN Kuiyou, WANG Han, HU De, WANG Zun, LIU Ning, ZHAO Liansheng. From Artificial to Intelligent:Research Progress of Individual Idendification Technology for Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1855-1866.
[3]	WANG Xueping, ZHANG Xiaojun, LI Xiaoyue, WANG Guodong, ZHANG Fuliang, SONG Yuwei, ZHANG Mingliang, MA Lei. Prokaryotic Expression of Hexon Protein of Highly Pathogenic Fowl Adenovirus Serotype 4 and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2053-2060.
[4]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[5]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[6]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[7]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[8]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[9]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[10]	XIAO Yangyang, LI Ruirui, MA Zhongchen, TANG Tian, WANG Na, CHEN Chuangfu, ZHENG Wei, WANG Yong, WANG Pengyan. Isolation,Identification and Drug Sensitivity Analysis of Mycoplasma bovis in Shihezi Region of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 754-763.
[11]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[12]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[13]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[14]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[15]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.