一株门多萨假单胞菌的分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2019.06.032

Abstract

Abstract:

The trial was designed to identify pathogens infected with Pelteobagrus fulvidraco and explore their resistance.The bacteria were isolated and identified by bacterial culture,physiological and biochemical identification and 16S rDNA sequence analysis.PCR method was used to amplify drug resistance genes.The resistance of drugs was detected by agar disk diffusion test.The results showed that the isolate was basically identical to Pseudomonas mendocina,and the 16S rDNA gene was 1 442 bp in length.Homology and phylogenetic tree analysis showed that the 16S rDNA sequence of this bacterium was 99.6% homologous to Pseudomonas mendocina NK-01,and their genetic relationship was the closest.The isolated strain was identified to be Pseudomonas mendocina,named as fsznc-01.The results of drug resistance gene test showed that the isolate fsznc-01 contained β-lactams resistance gene TEM,aminoglycosides resistance genes aph(3')-Ⅱa,aac(6)-Ⅰb and aac(3)-Ⅱa,and the sulfonamide resistance genes Sul1 and Sul2,and sulfonamide resistance genes Sul3 was not detected.The results of drug susceptibility test showed that the isolate fsznc-01 was sensitive to ceftazidime,gentamicin and piperacillin,and was resistant to cephalexin,cefazolin and cefradine.The results of this test could provide a reference for the prevention of fish diseases caused by Pseudomonas mendocina infection.

Key words: Pseudomonas mendocina; isolation and identification; drug resistance gene; drug susceptibility analysis; Pelteobagrus fulvidraco

CLC Number:

S943

ZHANG Nanchi, FANG Qing, WANG Li. Isolation,Identification and Drug Susceptibility Analysis of a Strain of Pseudomonas mendocina[J]. , 2019, 46(6): 1832-1840.

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Isolation,Identification and Drug Susceptibility Analysis of a Strain of Pseudomonas mendocina

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