2017年江西部分地区PEDV分子流行病学调查

doi:10.16431/j.cnki.1671-7236.2019.05.026

Abstract

Abstract:

To investigate the epidemiological and genetic variation of porcine epidemic diarrhea virus (PEDV) in Jiangxi province,a total of 182 clinical porcine intestinal and fecal samples were collected from pig farms in some areas of Jiangxi province in 2017 and detected for PEDV by RT-PCR.Two pairs of primers were designed and the S gene from 37 PEDV positive samples were amplified,cloned and sequenced.Genetic evolution analysis of S gene was performed using bioinformatics software and referenced PEDV strains registered in GenBank.The results showed that the length of S gene genome of 37 Jiangxi PEDV strains was 4 158/4 161 bp,encoding 1 385/1 386 amino acids.All PEDV strains belong to Group 1 which had a close relationship with the United States strains,and differed genetically from European strains (Br1/87) and the vaccine strains (CV777).36 of 37 PEDV Jiangxi strains were G1-1 and 1 strain was G1-2.The nucleotides homology of S gene among 37 PEDV strains were 96.9% to 100.0%,and that of amino acids homology was 96.1% to 100.0%.The nucleotides and amino acids homology of S gene between PEDV strains and referenced PEDV strains were 92.7% to 100.0% and 91.5% to 100.0%,respectively.Compared with the vaccine strains (CV777),S gene of PEDV Jiangxi strains existed bases deletions,insertions and mutation.This study confirmed the prevalence and variation of PEDV in Jiangxi province from the perspective of molecular epidemiology,and provided references for guiding the scientific prevention and control of PEDV in Jiangxi province.

Key words: porcine epidemic diarrhea virus (PEDV); molecular epidemiology; S gene; genetic variation

CLC Number:

S852.65

JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, ZHANG Zhiqing, CHEN Songchang, DENG Shunzhou. Molecular Epidemiological Investigation of Porcine Epidemic Diarrhea Virus in Jiangxi Province in 2017[J]. , 2019, 46(5): 1474-1482.

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Molecular Epidemiological Investigation of Porcine Epidemic Diarrhea Virus in Jiangxi Province in 2017

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