›› 2018, Vol. 45 ›› Issue (6): 1715-1721.doi: 10.16431/j.cnki.1671-7236.2018.06.037

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The Establishment of the Intestinal Injury Model of PEDV Infection in Young Piglets

LI Peng1, WU Mengjun1, YU Kui1, XU Haiwang1, WU Tao1,2, YI Dan1,2, WANG Lei1,2, ZHAO Di1,2, HOU Yongqing1,2   

  1. 1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;
    2. Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China
  • Received:2017-09-18 Online:2018-06-20 Published:2018-06-15

Abstract:

This study was conducted to establish the intestinal injury model of porcine epidemic diarrhea virus (PEDV) infection in neonatal piglets.Sixteen crossbred healthy piglets (Ducoc×Landrace×Yorkshire,7-day-old neonatal pigs) were randomly allocated to control and PEDV groups.During a 10-day trial,all the pigs were fed the basal diet.On the 7th day,the infected pigs were orally administrated with 104.5TCID50 of PEDV per pig,while the control pigs were given equal amount of sterile saline.On the 10th day,all pigs were orally gavaged with D-xylose (0.1 g/(kg·BW)).One hour later,blood was collected from jugular vein,and then all pigs were killed to obtain the small intestines.The average daily weight gain (ADG),diarrhea rate (DR),intestinal morphological structure,mucosal injury correlated gene mRNA level were measured.The results showed that:①PEDV infection increased the incidence of diarrhea (P<0.05),while reduced ADG (P<0.01);②PEDV infection decreased the plasma D-xylose content,villus height in the jejunum and ileum,the ratio of villus height to crypt depth in the duodenum,jejunum and ileum (P<0.01),and increased crypt depth in the duodenum and jejunum(P<0.05);③PEDV infection up-regulated the mRNA level of PEDV M gene in the jejunum (P<0.01),and down-regulated mRNA levels of villin and i-FABP in the jejunum (P<0.01).In conclusion,the intestinal injury model was successfully established with PEDV infection in neonatal piglets.

Key words: porcine epidemic diarrhea virus; piglets; intestinal injury; model

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