浙江省猪流行性腹泻病毒S1基因克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2018.06.031

Abstract

Abstract:

To analyze the genetic variation of porcine epidemic diarrhea virus (PEDV),the piglet diarrhea samples from Zhejiang province during 2015 to 2016 were detected by Real-time RT-PCR.Two pairs of primers were designed and the S1 gene of 16 PEDV positive samples from different regions were amplified by RT-PCR,cloned and sequenced.The sequence of S1 gene was analyzed by biological information software.The results showed that 48 samples were positive for PEDV.Homologous analysis showed that the S1 gene of 16 PEDV strains shared 93.1% to 99.8% nucleotide homology and 92.4% to 99.7% amino acid homology.The S1 gene of 16 PEDV strains shared 92.3% to 95.7% nucleotide homology and 90.7% to 95.7% amino acid homology with vaccine strain CV777.Compared to vaccine strain CV777,15 nucleotides were inserted and 6 nucleotides were absent in the S1 gene of 15 epidemic strains.The phylogenetic tree based on sequences of S1 gene suggested that 15 PEDV strains had a close relationship with the genotype Ⅱ PEDV,nucleotide and amino acid homologous were all more than 96.6%.Compared with the early separation of CV777 and LZC strains,nucleotide and amino acid homologous were all less than 93.4%.However,strain ZJ16NB6 had a close relationship with the S-INDEL-like strain,nucleotide and amino acid homologous were all 98.4% to 99.5%.The results indicated that PEDV infection was the main pathogen of piglets diarrhea in Zhejiang province.The genotype Ⅱ and S-INDEL-like PEDV strain existed in Zhejiang province,but mainly prevailing the genotype Ⅱ PEDV strains.

Key words: porcine epidemic diarrhea virus (PEDV); S1 gene; sequence analysis

CLC Number:

S852.65

XIE Ronghui, ZHAO Lingyan, NI Baifeng, CHAI Juan, WANG Yating, YU Yicong, XU Hui. Cloning and Sequence Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus from Zhejiang Province[J]. , 2018, 45(6): 1668-1676.

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Cloning and Sequence Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus from Zhejiang Province

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