›› 2018, Vol. 45 ›› Issue (2): 400-408.doi: 10.16431/j.cnki.1671-7236.2018.02.014

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Effect of Different Ratios of Crop Stalks and Alfalfa on Associative Effects of Diets in vitro

YUAN Jiu1, WANG Junjun1, YU Haishan1, PING Liying1, QI Peng1, WANG Dandan1, MA Guishan1, WAN Xinjie2   

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Lanzhou Federal Feed Limited Company, Lanzhou 730060, China
  • Received:2017-08-02 Online:2018-02-20 Published:2018-02-10

Abstract:

This experiment was conducted to investigate the effects of different ratios of crop stalks (potato vine, buckwheat straw, soybean straw, wheatgrass) and alfalfa on the associative effects (AE) of diets in vitro. Concentrate supplement:straw:alfalfa were 40:60:0, 40:50:10, 40:40:20, 40:30:30, 40:20:40, 40:10:50 and 40:0:60,while the concentrate-roughage ratio (C:R) was 40:60. Gas production (GP) was recorded at 0, 2, 4, 6, 9, 12, 24, 36, 48, 72 and 96 h. The AE was defined as the difference between the observed in vitro GP24 h and the predicted value from individual feed fermented alone. The results showed as follows:For potato vine, the AE of 0, 10, 30 potato vine groups were greater, and the AE of 0 potato vine group was significantly greater than 50 potato vine group (P < 0.05). The AE of all groups of both buckwheat straw and soybean straw were not significantly different (P > 0.05). For buckwheat straw, the AE of 20, 30, 40, 50 buckwheat straw groups were greater according to gas parameters in vitro, especially 40 buckwheat straw group. For soybean straw, the AE of 20, 30, 40, 50, 60 soybean straw groups were greater, especially the group of 50 soybean straw group. For wheatgrass, the AE of 10, 20 wheatgrass groups were greater, and they were extremely significantly greater than 50 and 60 wheatgrass groups (P < 0.01), 10 wheat grass group was significantly greater than 40 wheatgrass group (P < 0.05), 30 wheatgrass group was extremely significantly greater than 60 wheatgrass group (P < 0.01), and 0 wheatgrass group was significantly greater than 60 wheatgrass group (P < 0.05). It was concluded that the AE would be better when high levels of alfalfa and low levels of potato vine (0, 10, 30) or wheatgrass (10, 20) were combined, and low levels of alfalfa and high levels of soybean straw (50) or buckwheat straw (40) were combined.

Key words: associative effects; potato vine; buckwheat straw; soybean straw; wheatgrass; alfalfa; concentrate supplement

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