猪流行性腹泻病毒CH/GX/2015/750A株的分离鉴定及全基因组序列分析

doi:10.16431/j.cnki.1671-7236.2018.01.023

Abstract

Abstract:

In order to obtain a porcine epidemic diarrhea virus (PEDV) isolate that could grow and propagate efficiently in cell culture, and to know the genome sequence of the isolate, virus isolation was conducted on Vero cells through inoculating the intestinal content of piglets with watery diarrhea from swine farms in Guangxi. The strain was identified by cytopathic effects (CPE) and RT-PCR. The complete genome sequence of the isolate was determined by the next generation sequencing technology. One PEDV strain named as CH/GX/2015/750A was successfully isolated from the intestinal content of a piglet with watery diarrhea. The isolate could grow efficiently in cell culture, it had been serially propagated for over 25 passages and typical CPE was observed. The infectious titer of the virus gradually increased and kept at 10^7.50 TCID₅₀/mL. The complete genome sequence of CH/GX/2015/750A was 28 038 bp in length. The nucleotide homologies with 22 reference strains were 96.8% to 99.8%, and with 99.8% nucleotide homology to YC2014 PEDV strain. The whole genome and S gene phylogenetic analysis revealed that PEDV CH/GX/2015/750A isolate belonged to Ⅱa subgroup, and closely related with YC2014 and PEDV-WS mutant strains. The results showed that the PEDV strain obtained in the present study was a variant strain.

Key words: porcine epidemic diarrhea virus (PEDV); isolation and identification; complete genome; sequence analysis

CLC Number:

S852.65

QIN Yibin, LU Bingxia, HE Ying, DUAN Qunpeng, LI Bin, CHEN Zhongwei, LIANG Jiaxing, ZHOU Yingning, SU Qianlian, JIANG Dongfu, ZHAO Wu. Isolation, Identification and Complete Genome Sequence Analysis of Porcine Epidemic Diarrhea Virus CH/GX/2015/750A[J]. , 2018, 45(1): 178-188.

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Isolation, Identification and Complete Genome Sequence Analysis of Porcine Epidemic Diarrhea Virus CH/GX/2015/750A

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