《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (8): 2423-2430.doi: 10.16431/j.cnki.1671-7236.2019.08.028

• 遗传繁育 • 上一篇    下一篇

皱皮香猪WIF1基因结构变异WIF1-I8-sv889的研究

刘晓丽1,2, 冉雪琴1,2, 刘畅1,2, 牛熙1,2, 黄世会1,2, 王嘉福1,2,3   

  1. 1. 贵州大学生命科学学院, 山地植物资源保护与保护种质创新教育部重点实验室, 贵阳 550025;
    2. 贵州大学动物科学学院, 贵阳 550025;
    3. 铜仁学院, 铜仁 554300
  • 收稿日期:2019-03-14 出版日期:2019-08-20 发布日期:2019-08-17
  • 通讯作者: 冉雪琴, 王嘉福 E-mail:xqran@gzu.edu.cn;jfwang@gzu.edu.cn
  • 作者简介:刘晓丽(1994-),女,云南昆明人,硕士生,研究方向:动物生物化学与分子生物学,E-mail:lxlxs502@sina.com
  • 基金资助:

    国家高技术研究发展计划(863计划)课题(2013AA102503);国家自然科学基金(31672390);贵州省"百"层次创新型人才项目(黔科合人才2016-4012);贵州省农业攻关项目(黔科合支撑[2017]2585、黔科合支撑[2017]2587、黔科合NY[2013]3073);贵州省科技创新人才团队项目(黔科合平台人才〔2019〕5615)

Study on Structural Variation WIF1-I8-sv889 of WIF1 Gene in Xiang Pig with Wrinkled Skin

LIU Xiaoli1,2, RAN Xueqin1,2, LIU Chang1,2, NIU Xi1,2, HUANG Shihui1,2, WANG Jiafu1,2,3   

  1. 1. Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, College of Life Science, Guizhou University, Guiyang 550025, China;
    2. College of Animal Science, Guizhou University, Guiyang 550025, China;
    3. Tongren University, Tongren 554300, China
  • Received:2019-03-14 Online:2019-08-20 Published:2019-08-17

摘要:

为探究WIF1(Wnt inhibitory factor 1)基因中WIF1-I8-sv889结构变异位点变异与皱皮香猪躯干被毛形成的关系,本试验采用冰冻组织切片观察皱皮香猪皮肤毛囊的组织学形态,采用PCR方法分析WIF1-I8-sv889位点在猪群中的分布频率,并通过在线软件UCSC、RegRNA 2.0对结构变异序列所含的功能元件进行分析。组织学研究显示,与正常香猪和大白猪皮肤相比,皱皮香猪毛囊毛根鞘伸入真皮层,形成棘突,且皱褶凹陷处毛囊聚集,相反皱褶凸起处毛囊较少;皱皮香猪皮肤毛囊中的毛球宽度显著增大(P<0.05),单个毛囊中的毛干数极显著增多(P<0.01)。在WIF1-I8-sv889结构变异断点两端设计特异性引物,扩增片段长1383 bp,与参考基因组比,1383 bp中889 bp为结构变异区间,缺失581 bp,倒置308 bp。群体分布结果显示,猪群中WIF1-I8-sv889位点呈现出丰富的多态性,检测到3种基因型:正常的Ⅱ型、杂合的DI型和缺失的DD型,皱皮香猪中未检测到Ⅱ型;与正常香猪和大白猪相比,皱皮香猪中D等位基因占优势(94.44%);经卡方检验,皱皮香猪D等位基因显著或极显著高于正常香猪和大白猪(P<0.05;P<0.01)。结果提示,WIF1基因中的WIF1-I8-sv889结构变异可能与皱皮香猪毛囊的形态发生有关。

关键词: 皱皮香猪; 毛囊; 结构变异; WIF1基因

Abstract:

To explore the relationship of the structure variation of WIF1-I8-sv889 in the WIF1 (Wnt inhibitory factor 1) gene and the feature of body hair formation, the histological morphology of hair follicle was observed by using the frozen section technology and histochemistry dyeing method.The frequency of WIF1-I8-sv889 locus was determined by PCR method, and the functional elements in structural variation regions were analyzed by the online software UCSC and RegRNA 2.0.Histological research showed that compared with the skin structures of Xiang pig and Large White pigs, Xiang pig with wrinkled skin hair follicles appeared hyperplasia, hair root sheath extends into the dermis, forming spinous processes, and in Xiang pig with wrinkled skin, there were more hair follicles in the wrinkled depression, but the hair follicles in the wrinkled convex part were relatively less;Statistics on the width of the hair bulb and the number of hair follicles, the width of hair bulbs significantly increased in the hair follicles of Xiang pig with wrinkled skin (P<0.05), the number of hair shafts in a single hair follicle extremely significantly increased (P<0.01).Specific primers were designed at both ends of the WIF1-I8-sv889 structural variation breakpoint, the amplified fragment long was 1 383 bp, compared with the reference genome, 889 bp in 1 383 bp was the SV interval, 581 bp deleted, and 308 bp inverted.The population distribution results showed that, the polymorphisms of WIF1-I8-sv889 locus was abundant in the pig groups, there presented three genotypes, including types of Ⅱ, DI and DD;No genotype Ⅱ was detected in the Xiang pig with wrinkled skin;Compared with the common Xiang pig and Large White pigs, the D allele was dominant in the Xiang pigs with wrinkled skin (94.44%);Its frequency was significantly or extremely significantly higher than that of the other two pig groups (P<0.05;P<0.01).The results suggested that the structural variation of WIF1-I8-sv889 of WIF1 gene might be related to the morphogenesis of hair follicles in Xiang pig with wrinkled skin.

Key words: Xiang pig with wrinkled skin; hair follicle; structural variation; WIF1 gene

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