岷县黑裘皮羊微卫星遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2019.08.021

摘要/Abstract

摘要：

试验旨在分析岷县黑裘皮羊群体内遗传多样性，筛选出理想的遗传标记，为岷县黑裘皮羊的选育保种提供理论依据。选取144只岷县黑裘皮羊，颈静脉采血并提取DNA，对24对微卫星引物进行PCR扩增，用毛细管电泳技术进行基因分型，计算其等位基因数、等位基因大小及频率、有效等位基因数、杂合度和多态信息含量。结果显示，24个位点共检测到210个等位基因，平均等位基因数为8.75；群体等位基因频率为0.0104~0.7396，等位基因片段大小为97~285 bp；有效等位基因数为1.7581~8.2433个；群体平均观测杂合度为0.4839；平均期望杂合度为0.6959；平均多态信息含量为0.6527。其中MAF70位点等位基因数、有效等位基因数、期望杂合度和多态信息含量最高；OarFCB128位点观测杂合度最高；OarAE129位点等位基因数最少，SRCRSP9位点期望杂合度、多态信息含量最低；OarFCB304位点观测杂合度最低。Hardy-Weinberg平衡分析结果表明，所选位点中有19个处于非平衡状态。结果表明，岷县黑裘皮羊的遗传背景复杂，群体内遗传多样性丰富，可为其遗传资源的评估和选育保种工作提供理论依据。

关键词: 岷县黑裘皮羊; 微卫星; 遗传多样性; 毛细管电泳

Abstract:

This study was aimed to analyze the genetic diversity of Minxian Black fur sheep, select the ideal genetic markers, and provide a theoretical basis for breeding and conservation.144 Minxian Black fur sheep were selected to collect blood from jugular vein for extracting DNA.24 pairs of microsatellite primers were amplified by PCR, and the genotypes were analyzed by capillary electrophoresis.The numbers of alleles, alleles size and frequency, numbers of effective alleles, heterozygosity and polymorphism information content were calculated.The results showed that a total of 210 alleles were found in 24 loci, the average number of alleles was 8.75;The population alleles frequency were 0.0104 to 0.7396, the alleles size were 97 to 285 bp;The numbers of effective alleles were 1.7581 to 8.2433;The average observed heterozygosity was 0.4839;The average expected heterozygosity was 0.6959;The average polymorphism information content was 0.6527.The numbers of alleles, numbers of effective alleles, expected heterozygosity and polymorphism information content at MAF70 locus were the highest;The observe heterozygosity at OarFCB128 locus were the highest;The numbers of alleles at OarAE129 locus and the observed heterozygosity at OarFCB304 locus were the lowest;The expected heterozygosity and polymorphism information content at SRCRSP9 locus were the lowest.The Hardy-Weinberg equilibrium analysis results showed that 19 loci devieted from Hardy-Weinberg equilibrium.The results indicated that the genetic background of Minxian Black fur sheep was complex and the genetic diversity was rich, which could provide a theoretical basis for the evaluation of genetic resources and the breeding and conservation.

Key words: Minxian Black fur sheep; microsatellite; genetic diversity; capillary electrophoresis

中图分类号:

S813.3

张瑞, 郎侠, 吴建平, 刘婷, 宫旭胤, 王彩莲, 迟浩斌, 梁婷玉. 岷县黑裘皮羊微卫星遗传多样性分析[J]. 《中国畜牧兽医》, 2019, 46(8): 2360-2370.

ZHANG Rui, LANG Xia, WU Jianping, LIU Ting, GONG Xuyin, WANG Cailian, CHI Haobin, LIANG Tingyu. Genetic Diversity Analysis of Microsatellite in Minxian Black Fur Sheep[J]. , 2019, 46(8): 2360-2370.

参考文献 27

[1]	王成强,张广.岷县黑裘皮羊品种资源现状及发展对策[J].畜牧兽医杂志,2016,35(6):76-77. WANG C Q,ZHANG G.Current situation and development countermeasure of Minxian Black fur sheep breed resources[J]. Journal of Animal Science and Veterinary Medicine,2016,35(6):76-77.(in Chinese)
[2]	刘伯河,王成强,张广.岷县黑裘皮羊生产方式调查[J].甘肃畜牧兽医,2018,48(4):38-39. LIU B H,WANG C Q,ZHANG G.Production method survey of Minxian Black fur sheep[J]. Gansu Animal Husbandry and Veterinary,2018,48(4):38-39.(in Chinese)
[3]	AL-ATIYAT R M.The power of 28 microsatellite markers for parentage testing in sheep[J]. Electronic Journal of Biotechnology,2015,18(2):116-121.
[4]	陈扣扣,杨博辉,郎侠,等.8个微卫星位点在甘肃高山细毛羊肉毛兼用品系中的遗传多样性研究[J].中国畜牧兽医,2009,36(2):77-81. CHEN K K,YANG B H,LANG X,et al.The study on genetic polymorphism of eight microsatellite loci in Gansu alpine fine wool sheep that used for both mutton and wool[J]. China Animal Husbandry & Veterinary Medicine,2009,36(2):77-81.(in Chinese)
[5]	赵索南,保善科,郎侠.8个微卫星座位在海北金银滩藏羊群体中的多态性分析[J].中国草食动物科学,2012,32(2):15-18. ZHAO S N,BAO S K,LANG X.Analysis on the genetic diversity of eight microsatellite loci in Jinyintan sheep[J]. China Herbivore Science,2012,32(2):15-18.(in Chinese)
[6]	周明亮,陈明华,庞倩,等.布拖黑绵羊微卫星标记遗传多样性研究[J].草学,2018,2:74-80. ZHOU M L,CHEN M H,PANG Q,et al.Polymorphic analysis of Butuo Black sheep with microsatellite marker[J]. Prataculture & Animal Husbandry,2018,2:74-80.(in Chinese)
[7]	朱兰,孙利民,梁家充,等.利用微卫星标记分析云南省10个山羊品种的遗传多样性[J].中国畜牧杂志,2019,55(1):57-63. ZHU L,SUN L M,LIANG J C,et al.Analysis of genetic diversity of 10 goat breeds in Yunnan province using microsatellite markers[J]. Chinese Journal of Animal Science,2019,55(1):57-63.(in Chinese)
[8]	LAWSON HANDLEY L J,BYRNE K,SANTUCCI F,et al.Genetic structure of European sheep breeds[J]. Heredity,2007,99(6):620-631.
[9]	JAWASREH K I,ABABNEH M M,ISMAIL Z B,et al.Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers[J]. Veterinary World,2018,11(6):778-781.
[10]	DAVID C M G,QUIRINO C R,VEGA W H O,et al.Diversity of indigenous sheep of an isolated popula-tion[J]. BMC Veterinary Research,2018,14(1):350.
[11]	邱小宇,黄勇富,赵永聚,等.中国6个地方绵羊品种的微卫星分析[J].中国畜牧兽医,2016,43(4):1024-1031. QIU X Y,HUANG Y F,ZHAO Y J,et al.Microsatellite analysis of six Chinese indigenous sheep breeds[J]. China Animal Husbandry & Veterinary Medicine,2016,43(4):1024-1031.(in Chinese)
[12]	郎侠,吕潇潇.岷县黑裘皮羊群体遗传结构的微卫星DNA多态性分析[J].黑龙江畜牧兽医,2010,17:49-52. LANG X,LYU X X.Microsatellite DNA polymorphism analysis of population genetic structure in Minxian Black fur sheep[J]. Heilongjiang Animal Science and Veterinary Medicine,2010,17:49-52.(in Chinese)
[13]	CRAWFORD A M,LITTLEJOHN R P.The use of DNA markers in deciding conservation priorities in sheep and other livestock[J]. Animal Genetic Resources Information,1998,23(23):21-26.
[14]	郎侠,吕潇潇.兰州大尾羊微卫星DNA多态性研究[J].中国畜牧杂志,2011,47(1):14-17. LANG X,LYU X X.Study on microsatellite DNA polymorphism of Lanzhou Big-tail sheep[J]. Chinese Journal of Animal Science,2011,47(1):14-17.(in Chinese)
[15]	樊月圆,余淑青,曹振辉,等.云南红骨圭山山羊微卫星DNA位点遗传多样性分析[J].云南农业大学学报(自然科学),2013,28(3):329-335. FAN Y Y,YU S Q,CAO Z H,et al.Microsatellite analysis of genetic diversity of Red-bone Guishan goats and common Guishan goats[J]. Journal of Yunnan Agricultural University(Natural Science),2013,28(3):329-335.(in Chinese)
[16]	MCLAREN R J,ROGERS G R,DAVIES K P,et al.Linkage mapping of wool keratin and keratin-associated protein genes in sheep[J]. Mammalian Genome,1997,8(12):938-940.
[17]	吕慎金.我国七个绵羊群体微卫星DNA的遗传多样性研究[D].杨凌:西北农林科技大学,2003. LYU S J.Genetic diversity analysis with microsatellite DNA on 7 sheep populations of China[D].Yangling:Northwest A & F University,2003.(in Chinese)
[18]	吕潇潇.甘肃省绵羊品种遗传多样性的微卫星分析[D].兰州:甘肃农业大学,2009. LYU X X.Analysis on genetic diversity in sheeep breeds in Gansu province by microsatellite markers[D].Lanzhou:Gansu Agricultural University,2009.(in Chinese)
[19]	包文斌,束婧婷,许盛海,等.样本量和性比对微卫星分析中群体遗传多样性指标的影响[J].中国畜牧杂志,2007,43(1):6-9. BAO W B,SHU J T,XU S H,et al.Effects of sample size and sex ratio on various genetic diversity measures with microsatellite markers[J]. Chinese Journal of Animal Science,2007,43(1):6-9.(in Chinese)
[20]	雒林通.甘肃高山细毛羊优质毛品系微卫星标记与经济性状相关性研究[D].兰州:甘肃农业大学,2009. LUO L T.Correlation analysis of microsatellite DNA markers with some substantial economic traits in high quality fine wool stain of Gansu alpine fine-wool sheep[D].Lanzhou:Gansu Agricultural University,2009.(in Chinese)
[21]	BAI J Y,JIA X P,YANG Y B,et al.Polymorphism analysis of Henan Fat-tailed sheep using microsatellite markers[J]. Journal of Animal & Plant Sciences,2014,24(3):965-968.
[22]	BOTSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics,1980,32(3):314-331.
[23]	PURVIS W I,FRANKLIN I R.Major genes and QTL influencing wool production and quality:A review[J]. Genetics Selection Evolution,2005,37(Suppl 1):S97-S107.
[24]	梁家充,朱兰,孙利民,等.云南地方绵羊品种遗传多样性分子评价[J].四川农业大学学报,2018,36(6):822-830. LIANG J C,ZHU L,SUN L M,et al.Molecular evaluation of genetic diversity of native sheep breeds in Yunnan province[J]. Journal of Sichuan Agricultural University,2018,36(6):822-830.(in Chinese)
[25]	马丽娜,马青,李颖康.滩羊12个微卫星基因座遗传多态性研究[J].畜牧与饲料科学,2018,39(4):26-31. MA L N,MA Q,LI Y K.Genetic polymorphisms of twelve microsatellite loci in Tan sheep[J]. Animal Husbandry and Feed Science,2018,39(4):26-31.
[26]	邹杰.暗纹东方鲀养殖群体遗传多样性分析及生长性状微卫星标记筛选[D].上海:上海海洋大学,2014. ZOU J.Studying genetic structure among cultured populations and screening growth-related DNA markers in Takifugu obscurus[D].Shanghai:Shanghai Ocean University,2014.(in Chinese)
[27]	郑基阳,陈乃富,王晖,等.霍山石斛微卫星标记的筛选及遗传多样性分析[J].中国中药杂志,2011,36(21):2926-2931. ZHENG J Y,CHEN N F,WANG H,et al.Isolation and analysis of polymorphic microsatellite loci in Dendrobium huoshanense[J]. China Journal of Chinese Materia Medica,2011,36(21):2926-2931.(in Chinese)