《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (8): 2346-2353.doi: 10.16431/j.cnki.1671-7236.2019.08.019
梁钰, 张坤, 王梦云, 赵新豪, 李春丽
收稿日期:
2019-01-23
出版日期:
2019-08-20
发布日期:
2019-08-17
通讯作者:
李春丽
E-mail:hnclli@163.com
作者简介:
梁钰(1995-),女,河南郑州人,硕士,研究方向:动物遗传育种,E-mail:1053983727@qq.com
基金资助:
国家自然科学基金项目(31101694);河南省高校创新人才支持计划(14HASTIT021)
LIANG Yu, ZHANG Kun, WANG Mengyun, ZHAO Xinhao, LI Chunli
Received:
2019-01-23
Online:
2019-08-20
Published:
2019-08-17
摘要:
β防御素是猪体内分泌的一类抗菌肽,广泛分布于各个组织中,在抵抗病原入侵和免疫调节中发挥着重要作用。猪β防御素对细菌、真菌和病毒都有很强的杀灭作用,并且具有分子量小、热稳定性好、无残留等优点,是理想的抗生素替代品。研究表明,猪β防御素的表达与个体抗病力正相关,增加内源性防御素表达或直接摄入外源性防御素均能增强机体免疫力并促进生长。脂肪酸、氨基酸、维生素、益生菌、病原微生物等外源刺激皆可影响β防御素的表达。随着防御素相关研究的深入,将有望解决养猪生产中长期使用抗生素而导致的耐药性、药物残留及环境污染等问题。文章介绍了猪β防御素的结构特征、表达特异性及生物学功能,主要综述了外源刺激对β防御素表达的影响及其作用机制,初步说明外源β防御素作为饲料添加剂在仔猪生产中的效果,旨在为猪β防御素的深入研究及其在生猪健康养殖中的应用提供参考。
中图分类号:
梁钰, 张坤, 王梦云, 赵新豪, 李春丽. 猪β防御素研究进展[J]. 《中国畜牧兽医》, 2019, 46(8): 2346-2353.
LIANG Yu, ZHANG Kun, WANG Mengyun, ZHAO Xinhao, LI Chunli. Research Advance on Procine β Defensins[J]. , 2019, 46(8): 2346-2353.
[1] | 单安山,田昊天,邵长轩,等.抗菌肽抗细菌机理研究进展[J].东北农业大学学报,2018,49(3):84-94. SHAN A S,TIAN H T,SHAO C X,et al.Research advance on antibacterial mechanism of antimicrobial peptides[J]. Journal of Northeast Agricultural University,2018,49(3):84-94.(in Chinese) |
[2] | KIM C,KAUFMANN S H E.Defensin:A multifunctional molecule lives up to its versatile name[J]. Trends in Microbiology,2006,14(10):428-431. |
[3] | SELSTED M E,OUELLETTE A J.Mammalian defensins in the antimicrobial immune response[J]. Nature Immunology,2005,6(6):551-557. |
[4] | YANG D,LIU Z H,TEWARY P,et al.Defensin participation in innate and adaptive immunity[J]. Current Pharmaceutical Design,2007,13(30):3131-3139. |
[5] | ZHANG G,HUA W,SHI J,et al.Molecular cloning and tissue expression of porcine β-defensin-1[J]. Febs Letters,1998,424(1-2):37-40. |
[6] | ZHANG G,ROSS C R,BLECHA.Porcine antimicrobial peptides:New prospects for ancient molecules of host defense[J]. Veterinary Research,2000,31(3):277-296. |
[7] | SANG Y,PATIL A A,ZHANG G,et al.Bioinformatic and expression analysis of novel porcine β-defensins[J]. Mammalian Genome,2006,17(4):332-339. |
[8] | CHOI M K,LE M T,NGUYEN D T,et al.Genome-level identification,gene expression,and comparative analysis of porcine β-defensin genes[J]. BMC Genet,2012,13(1):98. |
[9] | ZHANG G,HIRAIWA H,YASUE H,et al.Cloning and characterization of the gene for a new epithelial beta-defensin.Genomic structure,chromosomal localization,and evidence for its constitutive expression[J]. Journal of Biological Chemistry,1999,274(34):24031-24037. |
[10] | 陈惠娴,毛若雨,滕达,等.二硫键及其连接方式对防御素抗菌功能的影响研究进展[J].生物技术通报,2016,32(9):32-37. CHEN H X,MAO R Y,TENG D,et al.Review on effects of disulfide bond and its connection on antimicrobial activity in defensins[J]. Biotechnology Bulletin,2016,32(9):32-37.(in Chinese) |
[11] | SHI J,ZHANG G,WU H,et al.Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations[J]. Infection Immunity,1999,67(6):3121-3127. |
[12] | 李美荃,刘红,张春勇,等.防御素基因在乌金猪与约大乌猪组织中的表达及东亚飞蝗抗菌活性物质对防御素基因表达的调节作用[J].动物营养学报,2016,28(7):2096-2105. LI M Q,LIU H,ZHANG C Y,et al.The expression of β-defensins genes in tissues of Wujin and Yuedawu pigs and effect of locust antibacterial active substances on expression of β-defensins genes[J]. Chinese Journal of Animal Nutrition,2016,28(7):2096-2105.(in Chinese) |
[13] | CHEN J,QI S,GUO R,et al.Different messenger RNA expression for the antimicrobial peptides beta-defensins between Meishan and crossbred pigs[J]. Molecular Biology Reports,2010,37(3):1633-1639. |
[14] | QI S,CHEN J,GUO R,et al.β-defensins gene expression in tissues of the crossbred and Tibetan pigs[J]. Livestock Science,2009,123(2-3):161-168. |
[15] | 王立刚,王立贤,程笃学.动物防御素研究进展[J].中国畜牧兽医,2007,34(3):85-89. WANG L G,WANG L X,CHENG D X.Advance in the research of animal defensin[J]. China Animal Husbandry & Veterinary Medicine,2007,34(3):85-89.(in Chinese) |
[16] | LI C L,ZHAO Y C,SONG X Y,et al.Molecular cloning,expression and characterization of the porcine β defensin 2 in E.coli[J]. Protein & Peptide Letters,2013,20(6):715-723. |
[17] | 姜丽华,卢海蓉,黄德新,等.猪β防御素1基因在毕赤酵母中的分泌表达[J].生物工程学报,2006,22(6):1036-1039. JIANG L H,LU H R,HUANG D X,et al.Expression of porcine β-defensin 1 gene in Pichia pastoris[J]. Chinese Journal of Biotechnology,2006,22(6):1036-1039.(in Chinese) |
[18] | PENG Z,WANG A,FENG Q,et al.High-level expression,purification,and characterisation of porcine β-defensin 2 in Pichia pastoris and its potential as a cost efficient growth promoter in porcine feed[J]. Applied Microbiology and Biotechnology,2014,98(12):5487-5497. |
[19] | YANG X,CHENG Y,TAN M,et al.Overexpression of porcine beta-defensin 2 enhances resistance to Actinobacillus pleuropneumoniae infection in pigs[J]. Infection Immunity,2015,83(7):2836-2843. |
[20] | VELDHUIZEN E J,RIJNDERS M,CLAASSEN E A,et al.Porcine beta-defensin 2 displays broad antimicrobial activity against pathogenic intestinal bacteria[J]. Molecular Immunology,2008,45(2):386-394. |
[21] | BALS R,WANG X,Z Z W,et al.Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung[J]. The Journal of Clinical Investigation,1998,102(5):874-880. |
[22] | GARCÍA J,KRAUSE A,SCHULZ S,et al.Human β-defensin 4:A novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity[J]. FASEB Journal,2001,15(10):1819-1821. |
[23] | MICHAELA W,BASTIAN K,DANIELA M,et al.Proteomic response of Bacillus subtilis to lantibiotics reflects differences in interaction with the cytoplasmic membrane[J]. Antimicrobial Agents Chemotherapy,2012,56(11):5749-5757. |
[24] | CHAN D I,PRENNER E J,VOGEL H J.Tryptophan-and arginine-rich antimicrobial peptides:Structures and mechanisms of action[J]. BBA-Biomembranes,2006,1758(9):1184-1202. |
[25] | PÁLFFY R,GARDLíK R,BEHULIAK M,et al.On the physiology and pathophysiology of antimicrobial peptides[J]. Molecular Medicine,2009,15(1-2):51-59. |
[26] | 薛现凤,韩菲菲,高彦华,等.猪β-防御素体外抗菌活性和抗氧化活性研究[J].农业生物技术学报,2012,20(11):1291-1299. XUE X F,HAN F F,GAO Y H,et al. In vitro detections of antimicrobial and antioxidant activities of porcine β-defensins[J]. Journal of Agricultural Biotechnology,2012,20(11):1291-1299.(in Chinese) |
[27] | CHEN R B,ZHANG K,ZHANG H,et al.Analysis of the antimicrobial mechanism of porcine beta defensin 2 against E.coli by electron microscopy and differentially expressed genes[J]. Scientific Reports,2018,8(1):14711. |
[28] | WAN M L,LING K H,WANG M F,et al.Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the production of antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells[J]. Molecular Nutrition & Food Research,2016,60(5):1048-1058. |
[29] | XUE M,ZHAO J,YING L,et al.IL-22 suppresses the infection of porcine enteric coronaviruses and rotavirus by activating STAT3 signal pathway[J]. Antiviral Research,2017,142:68-75. |
[30] | ALIZADEH A,AKBARI P,DIFILIPPO E,et al.The piglet as a model for studying dietary components in infant diets:Effects of galacto-oligosaccharides on intestinal functions[J]. British Journal of Nutrition,2015,115(4):605-618. |
[31] | CHENG Q,JIANG Z,XU C,et al.CpG oligodeoxynucleotide promotes protective immunity in the enteric mucosa and suppresses enterotoxigenic E.coli in the weaning piglets[J]. International Immunopharmacology,2010,10(10):1249-1260. |
[32] | MAO X,QI S,YU B,et al.Zn(2+) and L-isoleucine induce the expressions of porcine β-defensins in IPEC-J2 cells[J]. Molecular Biology Reports,2013,40(2):1547-1552. |
[33] | 蒙丹丽,梁列新,宋怀宇.短链脂肪酸在肠道中的生理作用[J].中国临床新医学,2018,11(2):198-202. MENG D L,LIANG L X,SONG H Y.Physiological function of short-chain fatty acids in the intestine[J]. Chinese Journal of New Clinical Medicine,2018,11(2):198-202.(in Chinese) |
[34] | ZENG X,SUNKARA L T,JIANG W,et al.Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs[J]. PLoS One,2013,8(8):e72922. |
[35] | XIONG H,GUO B,GAN Z,et al.Butyrate upregulates endogenous host defense peptides to enhance disease resistance in piglets via histone deacetylase inhibition[J]. Scientific Reports,2016,6:27070. |
[36] | WANG J,HUANG N,XIONG J,et al.Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase inhibition[J]. International Immunopharmacology,2018,65:303-311. |
[37] | 陈代文,毛湘冰,余冰,等.猪抗病营养研究进展[J].动物营养学报,2014,26(10):2992-3002. CHEN D W,MAO X B,YU B,et al.Recent advance in swine disease-resistant nutrition[J]. Chinese Journal of Animal Nutrition,2014,26(10):2992-3002.(in Chinese) |
[38] | MAN R,ZHANG S,LIU X,et al.Different lipopolysaccharide branched-chain amino acids modulate porcine intestinal endogenous β-defensin expression through the Sirt1/ERK/90RSK pathway[J]. Journal of Agricultural Food Chemistry,2016,64(17):3371-3379. |
[39] | 张曼,金鑫,田巧珍,等.益生菌对肠道先天防御反应调节作用的研究进展[J].中国畜牧兽医,2017,44(12):3656-3662. ZHANG M,JIN X,TIAN Q Z,et al.Advances in modulation of intestinal innate defense responses by probiotic bacteria[J]. China Animal Husbandry & Veterinary Medicine,2017,44(12):3656-3662.(in Chinese) |
[40] | LIU H,HOU C,WANG G,et al. Lactobacillus reuteri I5007 modulates intestinal host defense peptide expression in the model of IPEC-J2 cells and neonatal piglets[J]. Nutrients,2017,9(6):559. |
[41] | WANG J,ZENG Y,WANG S,et al.Swine-derived probiotic Lactobacillus plantarum inhibits growth and adhesion of enterotoxigenic Escherichia coli and mediates host defense[J]. Front Microbiol,2018,9:1364. |
[42] | LI X Q,ZHU Y H,ZHANG H F,et al.Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea:Intestinal microbiota and immune imbalances[J]. PLoS One,2012,7(7):e40666. |
[43] | JUN D,YUNFENG L,JINHUA Z,et al.Co-administration of Bacillus subtilis RJGP16 and Lactobacillus salivarius by strongly enhances the intestinal mucosal immunity of piglets[J]. Research in Veterinary Science,2013,94(1):62-68. |
[44] | WANG J,JI H,WANG S,et al.Probiotic Lactobacillus plantarum promotes intestinal barrier function by strengthening the epithelium and modulating gut microbiota[J]. Front Microbiol,2018,9:1953. |
[45] | COLLADO-ROMERO M,ARCE C,RAMI'REZ-BOO M A,et al.Quantitative analysis of the immune response upon Salmonella Typhimurium infection along the porcine intestinal gut[J]. BMC Veterinary Research,2010,41(2):23. |
[46] | VELDHUIZEN E J,HENDRIKS H G,HOGENKAMP A,et al.Differential regulation of porcine β-defensins 1 and 2 upon Salmonella infection in the intestinal epithelial cell line IPI-2I[J]. Veterinary Immunology and Immunopathology,2006,114(1-2):94-102. |
[47] | VELDHUIZEN E J,VAN DIJK A,TERSTEEG M H,et al.Expression of β-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig:Lack of upregulation in vivo upon Salmonella Typhimurium infection[J]. Molecular Immunology,2007,44(4):276-283. |
[48] | DAUDELIN J F,LESSARD M,BEAUDOIN F,et al.Administration of probiotics influences F4(K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs[J]. Veterinary Research,2011,42(1):69. |
[49] | SUN X,XIU F,PAN B,et al.Antimicrobial peptide expression in swine granulosa cells in response to Lipopolysaccharide[J]. Theriogenology,2018,119:80-90. |
[50] | ZHU C,WU Y,JIANG Z,et al.Dietary soy isoflavone attenuated growth performance and intestinal barrier functions in weaned piglets challenged with lipopolysaccharide[J]. International Immunopharmacology,2015,28(1):288-294. |
[51] | WAN M L,WOO C S,ALLEN K J,et al.Modulation of porcine beta-defensins 1 and 2 upon individual and combined fusarium toxin exposure in a swine jejunal epithelial cell line[J]. Applied and Environmental Microbiology,2013,79(7):2225-2232. |
[52] | WANG S,YANG J,ZHANG B,et al.Deoxynivalenol impairs porcine intestinal host defense peptide expression in weaned piglets and IPEC-J2 cells[J]. Toxins,2018,10(12):541. |
[53] | TANG Z,XU L,SHI B,et al.Oral administration of synthetic porcine beta-defensin-2 improves growth performance and cecal microbial flora and down-regulates the expression of intestinal toll-like receptor-4 and inflammatory cytokines in weaned piglets challenged with enterotoxigenic Escherichia coli[J]. Animal Science Journal,2016,87(10):1258-1266. |
[54] | XU J,ZHONG F,ZHANG Y,et al.Construction of Bacillus subtilis strain engineered for expression of porcine β-defensin-2/cecropin P1 fusion antimicrobial peptides and its growth-promoting effect and antimicrobial activity[J]. Asian-Australasian Journal of Animal Sciences,2016,30(4):576-584. |
[55] | PENG Z,WANG A,XIE L,et al.Use of recombinant porcine β-defensin 2 as a medicated feed additive for weaned piglets[J]. Scientific Reports,2016,6:26790. |
[56] | HAN F,ZHANG H,XIA X,et al.Porcine β-defensin 2 attenuates inflammation and mucosal lesions in dextran sodium sulfate-induced colitis[J]. Journal of Immunology,2015,194(4):1882-1893. |
[57] | 郭海勇,袁洪兴,王云霄,等.重组猪β-防御素1对大肠杆菌的抑制作用[J].畜牧兽医学报,2017,48(7):1342-1348. GUO H Y,YUAN H X,WANG Y X,et al.Inhibitory Effects of recombinant porcine beta-defensin 1 on Escherichia coli[J]. Chinese Journal of Animal and Veterinary Sciences,2017,48(7):1342-1348.(in Chinese) |
[58] | ELAHI S,BUCHANAN R,ATTAH-POKU S,et al.The host defense peptide beta-defensin 1 confers protection against Bordetella pertussis in newborn piglets[J]. Infection Immunity,2006,74(4):2338-2352. |
[1] | 韦明邦, 边巴琼达, 肖青青, 段梦琪, 强巴央宗, 商鹏. 藏猪CDKN1B基因克隆、生物信息学及表达分析[J]. 中国畜牧兽医, 2023, 50(6): 2196-2206. |
[2] | 吴旋, 曾申明. 血清白蛋白的功能、应用及纯化工艺研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2265-2275. |
[3] | 汪阳阳, 秦士贞, 唐德富, 邵玉新, 史兆国, 王铮, 李星, 赵东栋. 锌、乳酸杆菌对感染沙门氏菌乳鸽免疫及抗氧化功能的影响[J]. 中国畜牧兽医, 2023, 50(6): 2276-2285. |
[4] | 肖鹏, 尚江华, 杨春艳, 李孟琪, 段安琴, 马小娅, 冯超, 黄晨茜, 张博, 周金陈, 韦科龙, 郑威, 郑海英. α-亚麻酸对水牛卵巢颗粒细胞体外培养的影响[J]. 中国畜牧兽医, 2023, 50(6): 2380-2387. |
[5] | 焦琳琳, 成玉婷, 吴庆国, 吴双, 吴植, 朱善元, 钱莺娟. 鸭坦布苏病毒Capsid蛋白原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2395-2402. |
[6] | 李灿, 李楚楚, 曾维, 张宁, 纪春晓, 陈韬. 猪RegⅢγ蛋白的重组表达及功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2414-2426. |
[7] | 韩冰, 汤思淇, 朱大帷, 李海玲, 冯亚莉, 苏超凡, 徐艺娜, 冷昊钰, 王永涛, 张月美, 太晓春, 张莹. 新型疫苗佐剂研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2460-2467. |
[8] | 宋越, 苏胜杰, 张帆, 白帆, 戴伶俐, 王娜, 王大伟, 杨茜雯, 赵世华, 张月梅. 溶血性曼氏杆菌截短型白细胞毒素的原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2479-2486. |
[9] | 欧云文, 潘琴, 汪洋, 代军飞, 任绍科, 张洋, 张杰. 猪细小病毒6型ORF2基因的截短表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2487-2495. |
[10] | 王浩睿, 禹保军, 杨嘉翊, 魏榕, 付希, 王川川, 张迪, 李德生, 蔡正云, 顾亚玲, 张娟. 静原鸡DERA基因生物信息学分析及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1764-1773. |
[11] | 潘鹏丞, 雷宗全, 李显, 胡湘云, 秦谦涛, 覃兆鲜, 关志惠, 陈宝剑, 谢炳坤. 陆川猪MYL2基因生物信息学分析、真核表达载体构建及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1796-1806. |
[12] | 陆辉, 蔡高峰, 武彩红, 邱树磊, 袁橙, 陈晓兰, 刘芸, 覃凯莉. 茶树菇多糖对小鼠脾脏淋巴细胞免疫调节的影响[J]. 中国畜牧兽医, 2023, 50(5): 1828-1835. |
[13] | 乔宏兴, 张菡, 赵圣振, 孙彦婷, 张晓静, 康宇, 边传周. 发酵杜仲叶对肉鸡生产性能、抗氧化性能和免疫相关基因表达的影响[J]. 中国畜牧兽医, 2023, 50(5): 1845-1854. |
[14] | 谢华亮, 雷小文, 李建军, 刘珍妮, 钟云平, 谭东海, 张强. 蚯蚓液对105~175日龄蛋鸡产蛋性能、血清生化指标、抗氧化指标及免疫相关指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1899-1906. |
[15] | 卓娜, 朱忠武, 李静, 胡世享, 王建昌, 林华, 陈朝林, 韩佃刚, 艾军, 陈培富. 猪源戊型肝炎病毒ORF2蛋白生物信息学分析及真核表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1959-1970. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||