单宁和氧化锌组合对断奶仔猪粪便菌群、血清抗氧化及肝脏功能指标的影响

doi:10.16431/j.cnki.1671-7236.2019.06.010

摘要/Abstract

摘要：

本试验旨在研究单宁和氧化锌不同水平组合对断奶仔猪粪便菌群及血清免疫、抗氧化和肝脏功能相关血液生化指标的影响。选用体重相近，25日龄杜×长×大断奶仔猪270头，随机分成9组，每组3个重复，每个重复10头猪。对照组饲喂基础日粮加高锌（3 000 mg/kg氧化锌）的日粮，试验1~8组（T1~T8）分别饲喂基础日粮添加以下氧化锌与单宁组合的日粮：（2 000+0）、（2 000+1 000）、（1 000+0）、（1 000+1 000）、（1 000+1 500）、（1 000+2 000）、（0+1 500）、（0+2 000） mg/kg。试验期30 d。分别于试验的第14与28天采集仔猪新鲜粪样，测定粪便中各细菌的数量。试验结束采集血样，测定血清免疫、抗氧化及肝脏功能相关指标。结果显示，试验第14天，各组断奶仔猪粪便大肠杆菌、乳酸菌和双歧杆菌数量均无显著差异（P>0.05）。试验第28天，T1、T6组断奶仔猪粪便乳酸菌数量极显著高于对照组（P<0.01），而双歧杆菌数量则显著高于对照组（P<0.05），但各组大肠杆菌数量均无显著差异（P>0.05）。各组断奶仔猪血清中IgG和IgA含量、MDA含量及GSH-Px活力均无显著差异（P>0.05）。但T5、T6、T7、T8组断奶仔猪血清GSH含量极显著高于对照组（P<0.01）。除了T2和T5组，其余各组猪血清谷草转氨酶活性均显著或极显著低于高锌对照组（P<0.05；P<0.01）。本试验结果表明，与单一使用高剂量氧化锌相比，单宁与氧化锌适量水平组合有利于乳酸菌及双歧杆菌的增殖、提高断奶仔猪抗氧化能力并对肝脏产生保护作用。

关键词: 单宁; 氧化锌; 断奶仔猪; 粪便菌群; 免疫; 抗氧化

Abstract:

This experiment was conducted to investigate the effects of combinations of tannin and zinc oxide on fecal microbiota,serum immunity,antioxidation and liver function-related blood biochemical parameters in weaned piglets.Two hundred and seventy weaned piglets of Duroc×Landrace×Yorkshire on 25-day-old with similar body weight were randomly divided into nine groups with three replicates per group and ten pigs per replicate.Piglets in the control group were fed a basal diet supplemented with 3 000 mg/kg zinc oxide.Piglets in experimental groups 1 to 8(T1-T8) were fed basal diets added with following combinations of zinc oxide and tannin:(2 000+0),(2 000+1 000),(1 000+0),(1 000+1 000),(1 000+1 500),(1 000+2 000),(0+1 500) and (0+2 000) mg/kg,respectively.The trial lasted for 30 d.Fresh feces samples of piglets were collected on the 14th and 28th day of the trial to determine the number of 3 pathogenic bacteria in the feces.Blood samples were taken at the end of the experiment to determine immunity,antioxidant and liver function indexes in serum.The results showed that there were no significant differences in the numbers of E.coli,Lactobacillus and Bacillus bifidus in feces of piglets among each group on the 14th day of the trial (P>0.05).On the 28th day of the trial,the numbers of Lactobacillus in feces of piglets in groups T1 and T6 were extremely significantly higher than that in the control group (P<0.01),and the numbers of Bacillus bifidus in feces of piglets in groups T1 and T6 were significantly higher than that in the control group (P<0.05).However,there was no significant difference in the number of E.coli between each group (P>0.05).There were no significant differences in serum IgG and IgA content,MDA content and GSH-Px activity between each group (P>0.05).However,GSH content in serum in groups T5,T6,T7,T8 were extremely significantly higher than that in the control group (P<0.01).Except for the T2 and T5 groups,AST activity in serum of the other groups was significantly or extremely significantly lower than that of the high zinc control group (P<0.05;P<0.01).This test showed that compared with the single use of high dose zinc oxide,the appropriate combination of tannin and zinc oxide was beneficial to Lactobacillus and Bacillus bifidus proliferation,antioxidant capacity improvement of the body and liver protection in weaned piglets.

Key words: tannin; zinc oxide; weaned piglets; fecal microflora; immunity; antioxidation

中图分类号:

S828

陶勇, 杨晓志, 王见悠, 徐斌, 任善茂, 孙清平. 单宁和氧化锌组合对断奶仔猪粪便菌群、血清抗氧化及肝脏功能指标的影响[J]. 《中国畜牧兽医》, 2019, 46(6): 1644-1651.

TAO Yong, YANG Xiaozhi, WANG Jianyou, XU Bin, REN Shanmao, SUN Qingping. Effects of Combinations of Tannin and Zinc Oxide on Fecal Microflora, Serum Antioxidant and Liver Function Indexes in Weaned Piglets[J]. , 2019, 46(6): 1644-1651.

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