低水平葡萄糖对奶牛乳腺上皮细胞促炎症因子表达的影响

doi:10.16431/j.cnki.1671-7236.2019.05.032

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1525-1531.doi: 10.16431/j.cnki.1671-7236.2019.05.032

低水平葡萄糖对奶牛乳腺上皮细胞促炎症因子表达的影响

张金友¹, 黄鹤², 李伟¹, 韩欢胜¹, 甘文平¹, 宋伟红¹, 卫喜明¹

1. 黑龙江省农垦科学院畜牧兽医研究所, 哈尔滨 150038;
2. 黑龙江省疾病预防控制中心, 哈尔滨 150030

收稿日期:2018-10-10 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: 卫喜明 E-mail:1354629797@qq.com
作者简介:张金友(1979-),男,河北沧州人,博士,副研究员,研究方向:动物遗传育种与生理机能,E-mail:zhangjinyou868@aliyun.com
基金资助:
国家奶牛产业技术体系哈尔滨农垦综合试验站（CARS-36）；规模化奶牛场均衡增产关键技术集成创新与示范（HNK135-04-01）

Effect of Low Level Glucose on Proinflammatory Cytokines Expression in Bovine Mammary Epithelial Cells

ZHANG Jinyou¹, HUANG He², LI Wei¹, HAN Huansheng¹, GAN Wenping¹, SONG Weihong¹, WEI Ximing¹

1. Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Land Reclamation Science, Harbin 150038, China;
2. Heilongjiang Provincial Center for Disease Control and Prevention, Harbin 150030, China

Received:2018-10-10 Online:2019-05-20 Published:2019-05-20

摘要/Abstract

摘要：

本研究旨在探讨低葡萄糖水平对奶牛乳腺上皮细胞中肿瘤坏死因子-α（TNF-α）、溶菌酶（LYZ）、诱导型一氧化氮合酶（iNOS）、白细胞介素-6（IL-6）、乳铁蛋白（LF）和白细胞介素-8（CXCL8）等促炎症因子mRNA表达的影响。采用酶消化法将奶牛乳腺上皮细胞分离纯化后，分别在含有0.25、1.00和4.50 mg/mL葡萄糖的完全培养液中培养24 h，采用实时荧光定量PCR技术检测细胞中相关基因的表达。结果表明，上述基因的mRNA相对表达量在各葡萄糖浓度之间没有明显差异（P>0.05）。其中TNF-α和LYZ在各浓度葡萄糖中的表达量均非常少，iNOS的表达量居中，而IL-6、LF和CXCL8在各浓度组中的表达量相对较高。试验结果提示，葡萄糖浓度的减少并没有直接影响乳腺上皮细胞中TNF-α、LYZ、iNOS、IL-6、LF和CXCL8 mRNA的基础性表达；乳腺上皮细胞在未受到感染的情况下，能够表达一定数量的iNOS、IL-6、LF和CXCL8，这些因子可以作为先天性免疫防御的储备，在受到感染的情况下能迅速应对病菌的入侵。

关键词: 奶牛; 乳腺上皮细胞; 葡萄糖; 炎症

Abstract:

The aim of this study was to investigate the effects of low level glucose on mRNA expression of tumor necrosis factor-α (TNF-α),lysozyme (LYZ),induced nitric oxide synthase (iNOS),interleukin-6 (IL-6),lactoferrin (LF) and interleukin-8 (CXCL8) in bovine mammary epithelial cells.After enzymatic digestion,the bovine mammary epithelial cells were isolated and purified,and then cultured in complete medium containing 0.25,1.00 and 4.50 mg/mL glucose respectively.The expressions of related genes in cells were detected by Real-time fluorescence quantitative PCR.The results showed that there were no significant differences in the relative mRNA expression of the above genes among three glucose groups (P>0.05).The expression levels of TNF-α and LYZ in each concentration group were very low.The expression levels of iNOS were in the middle.However,the expression of IL-6,LF and CXCL8 in different concentration groups was relatively high.It suggested that the decrease of glucose concentration did not directly affect the basic expressions of TNF-α,LYZ,iNOS,IL-6,LF and CXCL8 mRNA in mammary epithelial cells.While without infection,mammary epithelial cells could express a certain amount of iNOS,IL-6,LF and CXCL8,which could be used as a reserve for innate immune defense in order to quickly respond to the invasion of pathogens.

Key words: bovine; mammary epithelial cells; glucose; inflammation

中图分类号:

S823

张金友, 黄鹤, 李伟, 韩欢胜, 甘文平, 宋伟红, 卫喜明. 低水平葡萄糖对奶牛乳腺上皮细胞促炎症因子表达的影响[J]. 《中国畜牧兽医》, 2019, 46(5): 1525-1531.

ZHANG Jinyou, HUANG He, LI Wei, HAN Huansheng, GAN Wenping, SONG Weihong, WEI Ximing. Effect of Low Level Glucose on Proinflammatory Cytokines Expression in Bovine Mammary Epithelial Cells[J]. , 2019, 46(5): 1525-1531.

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低水平葡萄糖对奶牛乳腺上皮细胞促炎症因子表达的影响

Effect of Low Level Glucose on Proinflammatory Cytokines Expression in Bovine Mammary Epithelial Cells

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