《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1525-1531.doi: 10.16431/j.cnki.1671-7236.2019.05.032

• 基础兽医 • 上一篇    下一篇

低水平葡萄糖对奶牛乳腺上皮细胞促炎症因子表达的影响

张金友1, 黄鹤2, 李伟1, 韩欢胜1, 甘文平1, 宋伟红1, 卫喜明1   

  1. 1. 黑龙江省农垦科学院畜牧兽医研究所, 哈尔滨 150038;
    2. 黑龙江省疾病预防控制中心, 哈尔滨 150030
  • 收稿日期:2018-10-10 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 卫喜明 E-mail:1354629797@qq.com
  • 作者简介:张金友(1979-),男,河北沧州人,博士,副研究员,研究方向:动物遗传育种与生理机能,E-mail:zhangjinyou868@aliyun.com
  • 基金资助:

    国家奶牛产业技术体系哈尔滨农垦综合试验站(CARS-36);规模化奶牛场均衡增产关键技术集成创新与示范(HNK135-04-01)

Effect of Low Level Glucose on Proinflammatory Cytokines Expression in Bovine Mammary Epithelial Cells

ZHANG Jinyou1, HUANG He2, LI Wei1, HAN Huansheng1, GAN Wenping1, SONG Weihong1, WEI Ximing1   

  1. 1. Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Land Reclamation Science, Harbin 150038, China;
    2. Heilongjiang Provincial Center for Disease Control and Prevention, Harbin 150030, China
  • Received:2018-10-10 Online:2019-05-20 Published:2019-05-20

摘要:

本研究旨在探讨低葡萄糖水平对奶牛乳腺上皮细胞中肿瘤坏死因子-α(TNF-α)、溶菌酶(LYZ)、诱导型一氧化氮合酶(iNOS)、白细胞介素-6(IL-6)、乳铁蛋白(LF)和白细胞介素-8(CXCL8)等促炎症因子mRNA表达的影响。采用酶消化法将奶牛乳腺上皮细胞分离纯化后,分别在含有0.25、1.00和4.50 mg/mL葡萄糖的完全培养液中培养24 h,采用实时荧光定量PCR技术检测细胞中相关基因的表达。结果表明,上述基因的mRNA相对表达量在各葡萄糖浓度之间没有明显差异(P>0.05)。其中TNF-α和LYZ在各浓度葡萄糖中的表达量均非常少,iNOS的表达量居中,而IL-6、LF和CXCL8在各浓度组中的表达量相对较高。试验结果提示,葡萄糖浓度的减少并没有直接影响乳腺上皮细胞中TNF-α、LYZ、iNOS、IL-6、LF和CXCL8 mRNA的基础性表达;乳腺上皮细胞在未受到感染的情况下,能够表达一定数量的iNOS、IL-6、LF和CXCL8,这些因子可以作为先天性免疫防御的储备,在受到感染的情况下能迅速应对病菌的入侵。

关键词: 奶牛; 乳腺上皮细胞; 葡萄糖; 炎症

Abstract:

The aim of this study was to investigate the effects of low level glucose on mRNA expression of tumor necrosis factor-α (TNF-α),lysozyme (LYZ),induced nitric oxide synthase (iNOS),interleukin-6 (IL-6),lactoferrin (LF) and interleukin-8 (CXCL8) in bovine mammary epithelial cells.After enzymatic digestion,the bovine mammary epithelial cells were isolated and purified,and then cultured in complete medium containing 0.25,1.00 and 4.50 mg/mL glucose respectively.The expressions of related genes in cells were detected by Real-time fluorescence quantitative PCR.The results showed that there were no significant differences in the relative mRNA expression of the above genes among three glucose groups (P>0.05).The expression levels of TNF-α and LYZ in each concentration group were very low.The expression levels of iNOS were in the middle.However,the expression of IL-6,LF and CXCL8 in different concentration groups was relatively high.It suggested that the decrease of glucose concentration did not directly affect the basic expressions of TNF-α,LYZ,iNOS,IL-6,LF and CXCL8 mRNA in mammary epithelial cells.While without infection,mammary epithelial cells could express a certain amount of iNOS,IL-6,LF and CXCL8,which could be used as a reserve for innate immune defense in order to quickly respond to the invasion of pathogens.

Key words: bovine, mammary epithelial cells, glucose, inflammation

中图分类号: