《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1447-1455.doi: 10.16431/j.cnki.1671-7236.2019.05.023

• 预防兽医 • 上一篇    下一篇

成都市猫泛白细胞减少症病毒、星状病毒和肠道冠状病毒的分子流行病学调查

张梦薇1, 陈艳1, 李妍2, 杨晓农1, 黄坚1   

  1. 1. 西南民族大学生命科学与技术学院, 成都 610041;
    2. 华派生物工程集团有限公司, 简阳 641400
  • 收稿日期:2018-11-16 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 黄坚 E-mail:huangjian.1122@163.com
  • 作者简介:张梦薇(1996-),女,四川成都人,学士,研究方向:动物疾病诊断,E-mail:272499920@qq.com
  • 基金资助:

    西南民族大学省级大学生创新创业大赛项目(S201710656022);国家重点研发计划项目(2017YFD0501101)

Molecular Epidemiological Investigation on FPV,FAstV and FECoV of Cat in Chengdu

ZHANG Mengwei1, CHEN Yan1, LI Yan2, YANG Xiaonong1, HUANG Jian1   

  1. 1. College of Life Sciences and Technology, Southwest Minzu University, Chengdu 610041, China;
    2. Huapai Bio-engineering Group Co., Ltd., Jianyang 641400, China
  • Received:2018-11-16 Online:2019-05-20 Published:2019-05-20

摘要:

为了解成都市区猫泛白细胞减少症病毒(FPV)、猫星状病毒(FAstV)和猫肠道冠状病毒(FECoV)的分子流行情况,本试验采集了315份家养猫和34份社区流浪猫的肛门拭子,含228份临床发病猫样本和121份临床健康猫样本,采用PCR方法对所有样本进行病毒核酸检测。同时,根据动物年龄、生活状态和免疫情况等因素进行病原流行分析,并根据病毒基因片段序列构建遗传进化树。结果显示,FPV、FAstV和FECoV的检出率分别为38.4%(134/349)、23.2%(81/349)和19.5%(68/349)。在混合感染方面,以FPV/FAstV (9.5%,33/349)、FAstV/FECoV (4.6%,16/349)和FPV/FECoV (4.3%,15/349)双重感染为主。基于部分基因序列的分析结果显示,样本间FPV VP2基因序列的同源性为95.1%~100%,发病猫毒株分为两个分支,其中一支与葡萄牙毒株(GenBank登录号:KT240136)和加拿大毒株(GenBank登录号:MF069445)遗传关系较近;另一支与中国的CPV毒株(GenBank登录号:KY937664)关系较近。样本间FAstV ORF1b基因序列同源性为90.9%~99.7%,发病猫的毒株的两个分支中一支与葡萄牙毒株(GenBank登录号:KF374704)和美国毒株(GenBank登录号:KM017743)关系较近;另一分支与中国香港毒株(GenBank登录号:KF499111)关系紧密。样本间FECoV的N基因序列同源性范围为89.3%~100%,与美国毒株(GenBank登录号:FJ938059)、意大利毒株(GenBank登录号:GU017092/GU017107)和中国毒株(GenBank登录号:KT852997)关系密切。调查结果表明,成都市区猫携带病毒性腹泻病原的情况较为普遍,部分猫存在多病原混合感染,且病毒基因序列存在明显变异,增加了病原流行及带毒猫发病的风险,需积极地进行临床防控。

关键词: 病毒性腹泻; 猫泛白细胞减少症病毒(FPV); 猫星状病毒(FAstV); 猫肠道冠状病毒(FECoV); 遗传进化

Abstract:

To understand the molecular prevalence of feline panleukopenia virus (FPV), feline astrovirus (FAstV) and feline enteric coronavirus (FEcoV) in Chengdu, this experiment collected 315 domestic cats and 34 community stray cats anal swabs containing 228 clinically infected cat samples and 121 clinical healthy cat samples. Viral nucleic acids in all samples were detected by PCR. At the same time, pathogen epidemiology analysis was carried out according to factors such as animal age, living state and immune status, and genetic evolution trees were constructed based on the sequences of viral gene fragments. The results showed that the detection rates of FPV, FAstV and FECoV were 38.4% (134/349), 23.2% (81/349) and 19.5% (68/349), respectively. In the case of mixed infection, FPV/FAstV (9.5%, 33/349), FAstV/FECoV (4.6%, 16/349) and FPV/FECoV (4.3%, 15/349) were the main infections. Based on the analysis of partial gene sequences, the homology of the FPV VP2 gene sequence between samples was 95.1% to 100%. The sick cat strains were divided into two branches, one of which was closely related to the Portuguese strain (GenBank accession No.:KT240136) and the Canadian strain (GenBank accession No.:MF069445), and the other was closely related to the Chinese CPV strain (GenBank accession No.:KY937664).The sequence homology of FAstV ORF1b gene between samples was 90.9% to 99.7%. One of the two branches of the sick cat strain was closely related to the Portuguese strain (GenBank accession No.:KF374704) and the American strain (GenBank accession No.:KM017743). The other branch was closely related to the Hong Kong strain of China (GenBank accession No.:KF499111).The N gene sequence homology of FECoV between samples was 89.3% to 100%, all the FECoV strains sequences were in a branch showing high homology to American (GenBank accession No.:FJ938059),Italy(GenBank accession No.:GU017092/GU017107) and Chinese strain (GenBank accession No.:KT852997).The survey results showed that the situation of cats carrying viral diarrhea in Chengdu was more common, some cats had multiple pathogen mixed infections, and the viral gene sequence had significant variation, increasing the risk of pathogen epidemics and the incidence of poisoned cats. It was necessary to actively carry out clinical prevention and control.

Key words: viral diarrhea, feline panleukopenia virus (FPV), feline astrovirus (FAstV), feline enteric coronavirus (FECoV), genetic variation

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