《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1551-1557.doi: 10.16431/j.cnki.1671-7236.2019.05.035

• 临床兽医 • 上一篇    下一篇

一例貉大肠杆菌与血虫混合感染的诊断鉴定

栾明明1,2, 王晓旭2, 贾赟3, 赵家平2, 张林波1, 徐超2, 韩鹏1   

  1. 1. 吉林农业大学生命科学学院, 长春 130118;
    2. 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建国家重点实验室, 长春 130112;
    3. 辽宁省出入境检验检疫局, 大连 116001
  • 收稿日期:2018-11-20 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 徐超, 韩鹏 E-mail:xuchao@caas.cn;hanpengjlau@163.com
  • 作者简介:栾明明(1992-),男,山东诸城人,硕士,研究方向:病原微生物,E-mail:790635101@qq.com
  • 基金资助:

    中国科协青年人才托举工程(2017QNRC001);吉林省优秀青年基金(20180520032JH);中央公益性科研院所基本科研业务费;吉林省教育厅"十三五"科学技术项目(JJKH20180674KJ);吉林农业大学博士启动基金(2015015)

Diagnosis and Identification of a Mixed Infection of Escherichia coli and Eperythrozoon in Raccoon Dog

LUAN Mingming1,2, WANG Xiaoxu2, JIA Yun3, ZHAO Jiaping2, ZHANG Linbo1, XU Chao2, HAN Peng1   

  1. 1. College of Life Science, Jilin Agricultural University, Changchun 130118, China;
    2. State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
    3. Liaoning Entry Exit Inspection and Quarantine Bureau, Dalian 116001, China
  • Received:2018-11-20 Online:2019-05-20 Published:2019-05-20

摘要:

为诊断吉林养殖场一例病死貉的病原,本试验运用细菌培养、染色镜检、生化鉴定、分子生物学鉴定和血液涂片镜检等方法对采集的病死貂的肺脏、肝脏、脾脏、肾脏、肠道及血液进行病原分离鉴定,并对分离到的病原菌进行致病性和药敏试验测定。结果显示,共分离出1株优势菌株,该分离菌株在光学显微镜下呈现为两端钝圆的杆状革兰氏阴性菌;生化试验结果显示,其能分解葡萄糖、乳糖、甘露醇、麦芽糖和蔗糖产酸产气,对于M-R试验、吲哚试验、淀粉水解试验结果为阳性;其16S rRNA序列与NCBI数据库中登录的已知大肠杆菌序列同源性达99%;动物致死性试验结果表明,该菌株对小鼠具有致病性;药敏试验结果显示,分离菌对喹诺酮类药物诺氟沙星和环丙沙星最敏感,对其他药物具有不同程度的耐药性;对病貉抗凝血进行涂片镜检证明有血虫感染,且红细胞感染率达98%以上。综上所述,该病貉感染的病原菌株为大肠杆菌且该大肠杆菌具有致病性,同时病貉患有血虫病,并导致了其生长发育迟缓。

关键词: 貉; 血虫体; 大肠杆菌; 诊断鉴定

Abstract:

To diagnose the pathogen of a diseased racoon dog in a Jilin farm,in this experiment,bacterial culture,staining microscopy,biochemical identification,molecular biology identification and blood smear microscopy were used to isolate and identify the pathogens of the collected lungs,liver,spleen,kidney,intestines and blood.And the isolated pathogens were tested for pathogenicity and susceptibility test.The results showed that a dominant strain was isolated,which was presented as a rod-shaped Gram-negative bacilli with obtuse ends at the optical microscope.The results of biochemical tests showed that it could decompose glucose-lactose,mannitol,maltose and sucrose to produce acid gas,which was positive for M-R test,sputum test and starch hydrolysis test.The 16S rRNA sequence had 99% homology with the known E.coli sequence registered in the NCBI database.The animal lethality test results showed that the strain was pathogenic to mice.The results of drug susceptibility test showed that the isolate was most sensitive to the quinolone drugs norfloxacin and ciprofloxacin,and had different degrees of resistance to other drugs.The anti-coagulation smears of sick racoon dog proved to be infected by Eperythrozoon,and the infection rate of red blood cells reached more than 98%.In summary,the pathogenic strain of the disease infection was E.coli and the E.coli was pathogenic,at the same time,the sick raccoon dog suffered from Eperythrozoon and led to its growth retardation.

Key words: racoon dog, Eperythrozoon, Escherichia coli, diagnosis and identification

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